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Titolo:
MYOBLAST-MEDIATED GENE-TRANSFER TO THE JOINT
Autore:
DAY CS; KASEMKIJWATTANA C; MENETREY J; FLOYD SS; BOOTH D; MORELAND MS; FU FH; HUARD J;
Indirizzi:
CHILDRENS HOSP PHILADELPHIA,DEPT ORTHOPAED SURG & MOL GENET & BIOCHEMPITTSBURGH PA 15261 CHILDRENS HOSP PHILADELPHIA,MUSCULOSKELETAL RES CTR,DEPT ORTHOPAED SURG PITTSBURGH PA 15261 UNIV PITTSBURGH,ATHLET DEPT,DIV SPORTS MED,MUSCULOSKELETAL RES CTR,DEPT ORTHOPAED SURG PITTSBURGH PA 15260
Titolo Testata:
Journal of orthopaedic research
fascicolo: 6, volume: 15, anno: 1997,
pagine: 894 - 903
SICI:
0736-0266(1997)15:6<894:MGTTJ>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
DUCHENNE MUSCULAR-DYSTROPHY; LONG-TERM EXPRESSION; ENGINEERED MYOBLASTS; MUSCLE-CELLS; IN-VIVO; TRANSPLANTATION; THERAPY; ARTHRITIS; MYOTUBES; MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
C.S. Day et al., "MYOBLAST-MEDIATED GENE-TRANSFER TO THE JOINT", Journal of orthopaedic research, 15(6), 1997, pp. 894-903

Abstract

Several genetic and acquired pathologic conditions of the musculoskeletal system, such as arthritis and damage to ligament, cartilage, and meniscus, may be amenable to gene therapy. Even though ex vivo gene transfer with synovial cells has been shown to deliver genes encoding for anti-arthritic proteins into the rabbit knee joint, its success has been limited by a transient transgene expression. In this study, data were investigated regarding the use of muscle cells as an alternative gene-delivery vehicle to the joint in newborn rabbit and adult severe combined immunodeficiency mice. We demonstrated that myoblasts were transduced more efficiently than synovial cells with use of the same adenoviral preparation ill vitro. After intra-articular injection, the engineered muscle cells adhered to several structures in the joint, including the ligament, capsule, and synovium. In addition, myoblasts fused to form many post-mitotic myotubes and myofibers at different locations of the joint of the newborn rabbit 5 days after the injection. In the knee of the adult mouse, myoblasts fused and expressed the reporter gene for at least 35 days after the injection. The presence of post-mitotic myofibers in the knee joint raises the possibility of long-term expression of the secreted protein. Currently, numerous tissues in the joint (ligament, meniscus, and cartilage) have poor intrinsic healing capacity and frequently need surgical corrections. A stable gene-delivery vehicle to the joint producing proteins that ameliorate these different musculoskeletal conditions may change the clinical implications of these pathologies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/08/20 alle ore 23:13:05