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Titolo:
ROLE OF RYANODINE RECEPTORS IN THE ASSEMBLY OF CALCIUM-RELEASE UNITS IN SKELETAL-MUSCLE
Autore:
PROTASI F; FRANZINIARMSTRONG C; ALLEN PD;
Indirizzi:
BRIGHAM & WOMENS HOSP,DEPT ANESTHESIA RES,120 SHATTUCK ST BOSTON MA 02115 BRIGHAM & WOMENS HOSP,DEPT ANESTHESIOL BOSTON MA 02115 UNIV PENN,DEPT CELL & DEV BIOL PHILADELPHIA PA 19104 CHILDRENS HOSP,DEPT CARDIOL,MOL CARDIOL LAB BOSTON MA 02115
Titolo Testata:
The Journal of cell biology
fascicolo: 4, volume: 140, anno: 1998,
pagine: 831 - 842
SICI:
0021-9525(1998)140:4<831:RORRIT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
JUNCTIONAL SARCOPLASMIC-RETICULUM; DIHYDROPYRIDINE RECEPTOR; TRANSVERSE TUBULES; 1,4-DIHYDROPYRIDINE RECEPTOR; CHARGE MOVEMENT; FOOT PROTEIN; ULTRASTRUCTURAL-LOCALIZATION; BIOCHEMICAL-CHARACTERIZATION; PERIPHERAL COUPLINGS; GLYCOPROTEIN TRIADIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
77
Recensione:
Indirizzi per estratti:
Citazione:
F. Protasi et al., "ROLE OF RYANODINE RECEPTORS IN THE ASSEMBLY OF CALCIUM-RELEASE UNITS IN SKELETAL-MUSCLE", The Journal of cell biology, 140(4), 1998, pp. 831-842

Abstract

In muscle cells, excitation-contraction (e-c) coupling is mediated by''calcium release units,'' junctions between the sarcoplasmic reticulum (SR) and exterior membranes, Two proteins, which face each other, are known to functionally interact in those structures: the ryanodine receptors (RyRs), or SR calcium release channels. and the dihydropyridine receptors (DHPRs), or L-type calcium channels of exterior membranes. In skeletal muscle, DHPRs form tetrads, groups of four receptors. and tetrads are organized in arrays that face arrays of feet (or RyRs), Triadin is a protein of the SR located at the SR-exterior membrane junctions, whose role is not known. We have structurally characterized calcium release units in a skeletal muscle cell line (1B5) lacking Ry(1)R. Using immunohistochemistry and freeze-fracture electron microscopy,we find that DHPR and triadin are clustered in foci in differentiating 1B5 cells. Thin section electron microscopy reveals numerous SR-exterior membrane junctions lacking foot structures (dyspedic). These results suggest that components other than Ry(1)Rs are responsible for targeting DHPRs and triadin to junctional regions. However, DHPRs in 1B5 cells are not grouped into tetrads as in normal skeletal muscle cells suggesting that anchoring to Ry(1)Rs is necessary for positioning DHPRs into ordered arrays of tetrads. This hypothesis is confirmed by finding a ''restoration of tetrads'' in junctional domains of surface membranes after transfection of 1B5 cells with cDNA encoding for Ry(1)R.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:37:14