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Titolo:
DIFFERENTIAL EXPRESSION OF ZPC IN THE BOVINE OVARY, OOCYTE, AND EMBRYO
Autore:
KOLLE S; SINOWATZ F; BOIE G; PALMA G;
Indirizzi:
UNIV MUNICH,INST TIERANAT 2,DEPT VET ANAT,VET STR 13 D-80539 MUNICH GERMANY BAYER FORSCHUNGSZENTRUM FORTPFLANZUNGSBIOL BADERFELD OBERSCHLIESSHE GERMANY
Titolo Testata:
Molecular reproduction and development
fascicolo: 4, volume: 49, anno: 1998,
pagine: 435 - 443
SICI:
1040-452X(1998)49:4<435:DEOZIT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORCINE ZONA-PELLUCIDA; TOTAL PROTEIN-SYNTHESIS; ACROSOME REACTION; INVITRO FERTILIZATION; SPERM RECEPTOR; MACROMOLECULAR-COMPOSITION; FOLLICULAR DEVELOPMENT; ALPHA-GLYCOPROTEIN; ABSOLUTE RATES; MOUSE OOCYTES;
Keywords:
BOVINE ZONA PELLUCIDA; IN VITRO FERTILIZATION; IN SITU HYBRIDIZATION; IMMUNOHISTOCHEMISTRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
S. Kolle et al., "DIFFERENTIAL EXPRESSION OF ZPC IN THE BOVINE OVARY, OOCYTE, AND EMBRYO", Molecular reproduction and development, 49(4), 1998, pp. 435-443

Abstract

Using nonradioactive in situ hybridization (ISH), the mRNA encoding the zona glycoprotein bZPC was localized in bovine ovaries, oocytes, and embryos. In the ovary, the distribution of the mRNA was correlated with the developmental stage of the follicle. Whereas in primordial andprimary follicles the mRNA was predominantly seen in the oocyte, it was found in both the oocyte and the follicle cells of secondary and tertiary follicles. In 2-day-old embryos produced by in vitro fertilization (IVF), no mRNA encoding ZPC could be demonstrated. Immunoblotting using monospecific polyclonal antibodies against porcine ZPC revealed a distinct band at a molecular weight of 47 kD in the ovarian cortex of cows, calves, and fetuses as well as in bovine follicle cells. Immunohistochemistry using the ZPC antibody displayed a strong signal in the zona pellucida of bovine oocytes and 2- to 6-day-old embryos as wellas in the follicle cells. Our results show that during follicular development bovine ZPC is synthesized by the oocyte of the primary follicle and by both the oocyte and the follicle cells of the secondary and tertiary follicle. After fertilization, the synthesis of the zona protein is finished. (C) 1998 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 15:23:32