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Titolo:
EFFECT OF LA-CROSSE VIRUS-INFECTION ON OVERWINTERING OF AEDES TRISERIATUS
Autore:
MCGAW MM; CHANDLER LJ; WASIELOSKI LP; BLAIR CD; BEATY BJ;
Indirizzi:
COLORADO STATE UNIV,ARTHROPOD BORNE & INFECT DIS LAB,COLL VET MED & BIOMED SCI,DEPT MICROBIOL FT COLLINS CO 80523 COLORADO STATE UNIV,ARTHROPOD BORNE & INFECT DIS LAB,COLL VET MED & BIOMED SCI,DEPT MICROBIOL FT COLLINS CO 80523 USA,MED RES INST INFECT DIS,DIV VIROL FREDERICK MD 21701
Titolo Testata:
The American journal of tropical medicine and hygiene
fascicolo: 2, volume: 58, anno: 1998,
pagine: 168 - 175
SICI:
0002-9637(1998)58:2<168:EOLVOO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
LACROSSE VIRUS; CULICIDAE; MOSQUITO; DIPTERA; RNA; DIAPAUSE; TERMINATION; TRANSCRIPTS; VECTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
M.M. Mcgaw et al., "EFFECT OF LA-CROSSE VIRUS-INFECTION ON OVERWINTERING OF AEDES TRISERIATUS", The American journal of tropical medicine and hygiene, 58(2), 1998, pp. 168-175

Abstract

The effect of La Crosse (LAC) virus infection on Aedes triseriatus overwintering success was determined. Eggs from LAC virus transovariallyinfected (LAG TOT+) and uninfected (LAG TOT-) Ae. triseriatus colonies were induced into diapause, held in natural conditions, and returnedto the laboratory at predetermined times for assay of diapause, mortality, and filial infection rates, and to examine viral transcription and replication during diapause. Embryos from the LAC TOT+ colony exhibited greater cumulative mortality (16.7%) than the LAC TOT-eggs (7.3%)throughout the overwintering periods. The increased mortality rate inLAC TOT+ eggs corresponded with a decrease in filial infection rates. Eggs from the LAC TOTS colony terminated diapause more readily than the LAC TOT-colony. An RNA strand-specific reverse transcriptase-polymerase chain reaction technique was used to monitor viral transcription and replication in mosquito eggs during overwintering, and to compare viral replication in diapausing and nondiapausing embryos. Viral messenger and replicative form RNA were present in eggs in all sample periods, suggesting that some virus replication occurred during diapause.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 17:52:31