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Titolo:
A SIMPLE DIPSTICK ASSAY FOR THE DETECTION OF ANTIBODIES TO PHENOLIC GLYCOLIPID-I OF MYCOBACTERIUM-LEPRAE
Autore:
BUHRER SS; SMITS HL; GUSSENHOVEN GC; VANINGEN CW; KLATSER PR;
Indirizzi:
ROYAL TROP INST,DEPT BIOMED RES,MEIBERGDREEF 39 NL-1105 AZ AMSTERDAM NETHERLANDS ROYAL TROP INST,DEPT BIOMED RES NL-1105 AZ AMSTERDAM NETHERLANDS FDN ADVANCEMENT PUBL HLTH & ENVIRONM PROTECT NL-3720 AL BILTHOVEN NETHERLANDS
Titolo Testata:
The American journal of tropical medicine and hygiene
fascicolo: 2, volume: 58, anno: 1998,
pagine: 133 - 136
SICI:
0002-9637(1998)58:2<133:ASDAFT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEPROSY PATIENTS; IGM ANTIBODIES; PHILIPPINES; CONTACTS; CEBU; ANTIGENS; ELISA; BSA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
S.S. Buhrer et al., "A SIMPLE DIPSTICK ASSAY FOR THE DETECTION OF ANTIBODIES TO PHENOLIC GLYCOLIPID-I OF MYCOBACTERIUM-LEPRAE", The American journal of tropical medicine and hygiene, 58(2), 1998, pp. 133-136

Abstract

Among the many reported applications of the detection of antibodies to phenolic glycolipid-I (PGL-I) of Mycobacterium leprae, in particular, the use of seroprevalence as an indicator of the magnitude of the leprosy problem may turn out to be very useful in leprosy control programs. An operational function of serology within the leprosy control services requires a simple test system. We have developed a simple dipstick assay for the detection of antibodies to PGL-I and compared its performance with that of an ELISA. A high degree of agreement (97.2%) wasobserved between the ELISA and the dipstick assay when tested on 435 sera; the agreement beyond chance (Kappa value) was 0.92. No significant difference was found between the dipstick assay and the ELISA when seropositivity rates obtained in groups of leprosy patients, householdcontacts, and controls were compared. The interpretation of the dipstick results as positive or negative was unequivocal, as illustrated bythe high agreement between different persons reading the test (Kappa values > 0.88). Storage of the only reagents required, the dipsticks and the stabilized detection reagent, up to three weeks under tropical conditions of high temperatures, high humidity, and exposure to light,did not influence the results of the assay. The dipstick assay described here is an easy-to-perform method for the detection of IgM antibodies to PGL-I of M. leprae; it does not require any special equipment and the highly stable reagents make the test robust and suitable for use in tropical countries. An internal control validates the performanceof the assay. This dipstick assay may be the method of choice for epidemiologic mapping of leprosy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 07:50:09