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Titolo:
CRYOPRESERVATION - AN ALTERNATIVE FOR THE LONG-TERM STORAGE OF OLD POTATO VARIETIES
Autore:
SCHAFERMENUHR A; MULLER E; MIXWAGNER G;
Indirizzi:
DSMZ,GERMAN COLLECT MICROORGANISMS & CELL CULTURES D-38124 BRAUNSCHWEIG GERMANY FAL,INST CROP SCI D-38116 BRAUNSCHWEIG GERMANY
Titolo Testata:
Potato research
fascicolo: 4, volume: 39, anno: 1996,
pagine: 507 - 513
SICI:
0014-3065(1996)39:4<507:C-AAFT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SHOOT-TIPS; SOLANUM-TUBEROSUM; LOW-TEMPERATURES; SURVIVAL; INVITRO; FREEZE; PRESERVATION; MERISTEMS; RECOVERY; CULTURES;
Keywords:
GENEBANK; GENETIC STABILITY; IN VITRO CULTURE; SHOOT-TIPS; SOLANUM TUBEROSUM L.;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
A. Schafermenuhr et al., "CRYOPRESERVATION - AN ALTERNATIVE FOR THE LONG-TERM STORAGE OF OLD POTATO VARIETIES", Potato research, 39(4), 1996, pp. 507-513

Abstract

A simple freezing method has been developed for the cryopreservation of an in vitro collection of old potato varieties. More than 125 varieties or genotypes have been frozen and stored in liquid nitrogen. Mostvarieties have high survival rates. averaging 80%. giant regenerationrates were lower and averaged 40%. Plants have been obtained from allvarieties or genotypes frozen and the phenotypes of regrown plants matched very well those of the unfrozen stock plants. However, one plantwas found which was probably polyploid and five plants had poor growth. In addition, the genetic stability of 161 regrown plants was checked by flow cytometric measurements and DNA-fingerprinting. In the 161 tested samples neither polyploid plants nor unusual banding patterns were found.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 07:45:26