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Titolo:
TREATMENT OF HUMAN SPERMATOZOA WITH SEMINAL PLASMA INHIBITS PROTEIN-TYROSINE PHOSPHORYLATION
Autore:
TOMES CN; CARBALLADA R; MOSES DF; KATZ DF; SALING PM;
Indirizzi:
UNIV NACL CUYO,CELLULAR & MOL BIOL LAB,INST HISTOL & EMBRIOL,FAC CIENCIAS MED,CASILLA CORREO 56 RA-5500 MENDOZA ARGENTINA DUKE UNIV,MED CTR,DEPT OBSTET & GYNECOL DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT CELL BIOL DURHAM NC 27710 CTR INVEST REPROD PEREZ COMPANC RA-1084 BUENOS AIRES DF ARGENTINA DUKE UNIV,MED CTR,DEPT BIOMED ENGN DURHAM NC 27710 DUKE UNIV,MED CTR,DEPT OBSTET & GYNECOL DURHAM NC 27710
Titolo Testata:
Molecular human reproduction
fascicolo: 1, volume: 4, anno: 1998,
pagine: 17 - 25
SICI:
1360-9947(1998)4:1<17:TOHSWS>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPERM MOTILITY INHIBITOR; HEPARIN-BINDING PROTEINS; MOUSE SPERM; RABBIT SPERMATOZOA; HYPERACTIVATED MOTILITY; CAPACITATION INVITRO; SUPEROXIDE-DISMUTASE; OXYGEN-CONSUMPTION; MEMBRANE-VESICLES; ACROSOME REACTION;
Keywords:
MOTILITY; PHOSPHORYLATION; SEMINAL PLASMA; SPERMATOZOA; TYROSINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
69
Recensione:
Indirizzi per estratti:
Citazione:
C.N. Tomes et al., "TREATMENT OF HUMAN SPERMATOZOA WITH SEMINAL PLASMA INHIBITS PROTEIN-TYROSINE PHOSPHORYLATION", Molecular human reproduction, 4(1), 1998, pp. 17-25

Abstract

It has long been known that seminal plasma contains factors that influence the fertilizing capacity of spermatozoa in many different ways. However, little is understood of the biochemical cascades triggered when spermatozoa and seminal plasma interact. In this study, we examinedhow incubation with seminal plasma affected protein tyrosine phosphorylation in human spermatozoa. Increased protein tyrosine phosphorylation is a hallmark of sperm capacitation in several mammalian species, including human. Seminal plasma blocks protein tyrosine phosphorylationwhen added to washed, non-capacitated spermatozoa. Removal of seminarplasma and incubation in capacitating medium led to partial recovery of the tyrosine phosphorylation cascade. Addition of seminal plasma toa suspension of spermatozoa previously incubated for 5 h under capacitating conditions decreased the lever of tyrosine phosphorylation on all proteins in a dose-dependent manner. In this case, the phosphotyrosine signal did not increase upon removal of seminal plasma followed byovernight incubation in fresh capacitating media, indicating that removal of seminal plasma was necessary but not sufficient for protein tyrosine phosphorylation to occur. These results indicate that human seminal plasma contains factors that influence the tyrosine phosphorylation status of human spermatozoa.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:29:04