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Titolo:
A PRACTICAL IN-VITRO SPERM-EGG BINDING ASSAY THAT DETECTS SUBFERTILE MALES
Autore:
BARBATO GF; CRAMER PG; HAMMERSTEDT RH;
Indirizzi:
PENN STATE UNIV,DEPT BIOCHEM & MOL BIOL,ALTHOUSE LAB 406 UNIVERSITY PK PA 16802 PENN STATE UNIV,DEPT BIOCHEM & MOL BIOL,ALTHOUSE LAB 406 UNIVERSITY PK PA 16802 PENN STATE UNIV,DEPT POULTRY SCI UNIVERSITY PK PA 16802
Titolo Testata:
Biology of reproduction
fascicolo: 3, volume: 58, anno: 1998,
pagine: 686 - 699
SICI:
0006-3363(1998)58:3<686:APISBA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERIVITELLINE LAYER; ACROSOME REACTION; EMBRYO QUALITY; ROOSTER SPERM; BOVINE SPERM; SPERMATOZOA; CRYOPRESERVATION; FERTILITY; MOTILITY; SEMEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
78
Recensione:
Indirizzi per estratti:
Citazione:
G.F. Barbato et al., "A PRACTICAL IN-VITRO SPERM-EGG BINDING ASSAY THAT DETECTS SUBFERTILE MALES", Biology of reproduction, 58(3), 1998, pp. 686-699

Abstract

A series of in vitro assays of sperm-egg binding were developed to identify potentially subfertile roosters, Initial assays used either segments of intact hen's egg perivitelline membrane (PVM) placed on a microscope slide or a heat-solubilized extract of PVM (MS-PVM) dried within a flat-bottomed microwell plate, with bound sperm detected with a DNA-specific stain and epifluorescence microscopy. An automated assay was developed using prestained inimidyl-7-amino-4-methylcoumarin-3-acetate-HS-PVM and enumeration of bound sperm with a fluorometric microwell plate reader, Four populations of chickens differing in fertility were evaluated with the following results: 1) the correlation across lines between in vitro sperm binding and fertility was 0.83 (N = 40; p < 0.0001); 2) correlations with other seminal parameters were low; and 3) the relationship between sperm binding and fertility was not linear,bur a threshold plot allowed identification of males with low bindingand low fertility Motile sperm from roosters, turkeys, buffs, humans,mice, rams, and stallions bound in a dose-responsive manner. Featuresof binding were revealed by both scanning and transmission electron microscopy. Use of this assay to cull males whose semen appears normal by traditional modes of analysis but differs in the obligatory trait of sperm-egg binding could be of value to avoid expensive progeny testing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 15:32:09