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Titolo:
TNF-ALPHA INCREASES ALBUMIN PERMEABILITY OF ISOLATED RAT GLOMERULI THROUGH THE GENERATION OF SUPEROXIDE
Autore:
MCCARTHY ET; SHARMA R; SHARMA M; LI JZ; GE XL; DILEEPAN KN; SAVIN VJ;
Indirizzi:
MED COLL WISCONSIN,FROEDTERT MEM LUTHERAN HOSP,9200 W WISCONSIN AVE MILWAUKEE WI 53226 MED COLL WISCONSIN,DEPT MED,DIV NEPHROL MILWAUKEE WI 53226 UNIV KANSAS,MED CTR,DEPT MED KANSAS CITY KS 66103
Titolo Testata:
Journal of the American Society of Nephrology
fascicolo: 3, volume: 9, anno: 1998,
pagine: 433 - 438
SICI:
1046-6673(1998)9:3<433:TIAPOI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; REACTIVE OXYGEN METABOLITES; HUMAN MESANGIAL CELLS; PLATELET-ACTIVATING-FACTOR; EPITHELIAL-CELLS; PUROMYCIN AMINONUCLEOSIDE; HYDROGEN-PEROXIDE; GENE-EXPRESSION; LUPUS NEPHRITIS; ELEVATED LEVELS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
E.T. Mccarthy et al., "TNF-ALPHA INCREASES ALBUMIN PERMEABILITY OF ISOLATED RAT GLOMERULI THROUGH THE GENERATION OF SUPEROXIDE", Journal of the American Society of Nephrology, 9(3), 1998, pp. 433-438

Abstract

Tumor necrosis factor-alpha (TNF-alpha) is a cytokine that plays a central role in inflammation. Glomerular levels of TNF-alpha are elevated in human and experimental glomerulonephritis. Glomerular cells produce and respond to TNF-alpha. One of the mechanisms by which these cells respond to TNF-alpha is through generation of reactive oxygen species. In this study, the effect of TNF-alpha on albumin permeability (P-albumin) of isolated rat glomeruli and the possible mechanism of this effect were examined. Isolated rat glomeruli were incubated with TNF-alpha (0.4 ng/ml), TNF-alpha with anti-TNF-alpha antibodies, and TNF-alpha with the reactive oxygen species scavengers superoxide dismutase, catalase, DMSO, or dimethylthiourea for 12 min at 37 degrees C, and P-albumin was calculated. TNF-alpha increased P-albumin of isolated glomeruli compared with control (0.70 +/- 0.02, n = 25 versus 0.00 +/- 0.05, n = 26), and this effect was abrogated by anti-TNF-alpha antibodies (-0.18 +/- 0.05, n = 23). Superoxide dismutase abolished the increase in P-albumin (-0.04 +/- 0.11, n = 23), whereas catalase (0.73 +/- 0.08, n = 30), DMSO (0.64 +/- 0.03, n = 10), or dimethylthiourea (0.51 +/-0.08, n = 10) did not alter the effect of TNF-alpha. These results indicate that TNF-alpha increased P-albumin of isolated glomeruli and that the mediator of the increased P-albumin is superoxide. It is concluded that TNF-alpha derived from glomerular or extraglomerular sources can increase glomerular P-albumin through generation of superoxide andmay lead to proteinuria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 06:16:36