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Titolo:
IN-SITU DETECTION OF AE2 ANION-EXCHANGER MESSENGER-RNA IN THE HUMAN LIVER
Autore:
GARCIA C; MONTUENGA LM; MEDINA JF; PRIETO J;
Indirizzi:
UNIV NAVARRA,DEPT INTERNAL MED E-31080 PAMPLONA SPAIN UNIV NAVARRA,DEPT INTERNAL MED E-31080 PAMPLONA SPAIN UNIV NAVARRA,UNIV CLIN & MED SCH,LIVER UNIT E-31080 PAMPLONA SPAIN UNIV NAVARRA,SCH MED,DEPT HISTOL & PATHOL E-31080 PAMPLONA SPAIN
Titolo Testata:
Cell and tissue research
fascicolo: 3, volume: 291, anno: 1998,
pagine: 481 - 488
SICI:
0302-766X(1998)291:3<481:IDOAAM>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-ERYTHROCYTE MEMBRANE; BAND 3-RELATED PROTEIN; CL-/HCO3 EXCHANGER; CDNA CLONING; EXPRESSION; LOCALIZATION; CELLS; GENE; SEQUENCE; MURINE;
Keywords:
ANION EXCHANGE; BICARBONATE SECRETION; BILE-DUCT EPITHELIAL CELLS; HEPATOCYTES; IMMUNOHISTOCHEMISTRY; LIVER LYMPHOCYTES; T CELLS; MAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
C. Garcia et al., "IN-SITU DETECTION OF AE2 ANION-EXCHANGER MESSENGER-RNA IN THE HUMAN LIVER", Cell and tissue research, 291(3), 1998, pp. 481-488

Abstract

Na+-independent anion exchangers, a family of membrane proteins that mediate electroneutral exchanges of chloride and bicarbonate ions across the cell membrane, are considered to be involved in intracellular pH regulation as well as in transepithelial acid/base transport. Previous immunohistochemical data have shown that anion-exchanger-2 (AE2) protein is expressed in the liver parenchyma, localizing at both the canaliculi and the luminal surfaces of intrahepatic bile ducts, where it may have a role in the biliary secretion of bicarbonate. In the present study, we have carried out in situ hybridization experiments on biopsies of human liver using three overlapping antisense anion-exchanger-2 riboprobes. Anion-exchanger-2 mRNA signals were localized mainly in the cytoplasm of terminal and interlobular bile-duct cells, whereas weaker signals were observed in bile-duct cells of larger intrahepatic ducts. Furthermore, some hepatocytes, mostly periportal, contained detectable anion-exchanger-2 mRNA signals in their cytoplasm. No hybridization signals were observed in controls with sense riboprobes, with omission of the antisense probe, or with treatment of the sections with RNase before hybridizations. Finally, intense anion-exchanger-2 hybridization signals were observed in lymphomononuclear cells in sinusoids and in portal infiltrates. Immunocytochemical data from reverse-phase sections suggest that these cells correspond to some of the CD45R+ (UCHL1+) T lymphocytes resident in the liver.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:57:00