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Titolo:
MUTATIONAL ANALYSIS OF THE DONOR SUBSTRATE-BINDING SITE OF THE RIBOSOMAL PEPTIDYLTRANSFERASE CENTER
Autore:
SAARMA U; SPAHN CMT; NIERHAUS KH; REMME J;
Indirizzi:
TARTU STATE UNIV,INST CELL & MOL BIOL,RIIA 23 EE-2400 TARTU ESTONIA TARTU STATE UNIV,INST CELL & MOL BIOL EE-2400 TARTU ESTONIA MAX PLANCK INST MOL GENET,AG RIBOSOMEN D-14195 BERLIN GERMANY
Titolo Testata:
RNA
fascicolo: 2, volume: 4, anno: 1998,
pagine: 189 - 194
SICI:
1355-8382(1998)4:2<189:MAOTDS>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PEPTIDYL TRANSFERASE CENTER; ESCHERICHIA-COLI; TRANSFER-RNA; CCA-END; NUCLEOTIDES; RESISTANCE; SUBUNIT; PROTEIN; MUTANT;
Keywords:
ESCHERICHIA COLI; 23S RIBOSOMAL-RNA MUTANTS; CMP STIMULATION; THIOSTREPTON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
U. Saarma et al., "MUTATIONAL ANALYSIS OF THE DONOR SUBSTRATE-BINDING SITE OF THE RIBOSOMAL PEPTIDYLTRANSFERASE CENTER", RNA, 4(2), 1998, pp. 189-194

Abstract

Previous experiments have shown that the top of helix 90 of 23S rRNA is highly important for the ribosomal peptidyltransferase activity andmight be part of the donor (P) site. Developing on these studies, mutations in the 23S rRNA at the highly conserved positions G2505, G2582,and G2583 were investigated. None of the mutations affected assembly,subunit association, or the capacity of tRNA binding to A and P sites. A ''selective transpeptidation assay'' revealed that the mutations specifically impaired peptide bond formation. Results with a modified ''fragment'' assay using the minimal donor substrate pA-fMet are consistent with a model where the nucleotides Psi GG2582 form a binding pocket for C75 of the tRNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 09:07:01