Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CHRONIC ETHANOL TREATMENT DECREASES [H-3]EPIBATIDINE AND [H-3]NICOTINE BINDING AND DIFFERENTIALLY REGULATES MESSENGER-RNA LEVELS OF NICOTINIC ACETYLCHOLINE-RECEPTOR SUBUNITS EXPRESSED IN M10 AND SH-SY5Y NEUROBLASTOMA-CELLS
Autore:
GORBOUNOVA O; SVENSSON AL; JONSSON P; MOUSAVI M; MIAO H; HELLSTROMLINDAHL E; NORDBERG A;
Indirizzi:
HUDDINGE UNIV HOSP,KAROLINSKA INST,DEPT CLIN NEUROSCI & FAMILY MED,DIV NICOTINE RES,B84 S-14186 HUDDINGE SWEDEN HUDDINGE UNIV HOSP,KAROLINSKA INST,DEPT CLIN NEUROSCI & FAMILY MED,DIV NICOTINE RES S-14186 HUDDINGE SWEDEN
Titolo Testata:
Journal of neurochemistry
fascicolo: 3, volume: 70, anno: 1998,
pagine: 1134 - 1142
SICI:
0022-3042(1998)70:3<1134:CETD[A>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
SHORT-SLEEP MICE; RAT-BRAIN; GENE-EXPRESSION; UP-REGULATION; CIGARETTE-SMOKING; CROSS-TOLERANCE; MESSENGER-RNAS; LONG-SLEEP; INHIBITION; ALCOHOL;
Keywords:
ETHANOL; [H-3]NICOTINE; [H-3]EPIBATIDINE; M10 CELLS; SH-SY5Y NEUROBLASTOMA CELLS; NICOTINIC RECEPTOR SUBTYPES; MESSENGER-RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
O. Gorbounova et al., "CHRONIC ETHANOL TREATMENT DECREASES [H-3]EPIBATIDINE AND [H-3]NICOTINE BINDING AND DIFFERENTIALLY REGULATES MESSENGER-RNA LEVELS OF NICOTINIC ACETYLCHOLINE-RECEPTOR SUBUNITS EXPRESSED IN M10 AND SH-SY5Y NEUROBLASTOMA-CELLS", Journal of neurochemistry, 70(3), 1998, pp. 1134-1142

Abstract

For a study of the underlying mechanisms of a possible interaction between ethanol and nicotinic receptors during ethanol dependence, the aim of this work was to investigate the effect of chronic ethanol exposure on nicotinic receptor subtypes in a transfected fibroblast cell line (M10 cells) stably expressing alpha 4 beta 2 nicotinic receptor subtype and an SH-SY5Y neuroblastoma cell line expressing alpha 3, alpha 5, alpha 7, beta 2, and beta 4 nicotinic acetylcholine receptor (nAChR) subunits. A significant dose-related decrease (-30-80%) in number of[H-3]nicotine binding sites was observed in ethanol-treated (25-240 mM) compared with untreated M10 cells, Similarly, 4-day treatment with ethanol in concentrations relevant to chronic alcoholism (100 mM) decreased the number of nicotinic receptor binding sites in the SH-SY5Y cells when measured using [H-3]epibatidine, When M10 cells were chronically treated with nicotine, ethanol partly inhibited the up-regulation of nicotinic receptors when present in the cells together with nicotine. Chronic treatment for 4 days with 100 mM ethanol significantly decreased the mRNA level for the alpha 3 nAChR subunit (-39%), while the mRNA levels for the alpha 7 (+30%) and alpha 4 (+22%) subunits were significantly increased. Chronic ethanol treatment did not affect the mRNA levels for the beta 2 nAChR subunit. Changes in the levels of nAChR protein and mRNA may have adaptive significance and be involved in thedevelopment of dependence, tolerance, and addiction to chronic ethanol and nicotine exposure. They also may be targets for therapeutic strategies in the treatment of ethanol and nicotine dependence.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 20:08:04