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Titolo:
ACQUIRED IMMUNE-RESPONSES TO THE N-TERMINAL AND C-TERMINAL REGIONS OFPLASMODIUM-VIVAX MEROZOITE SURFACE PROTEIN-1 IN INDIVIDUALS EXPOSED TO MALARIA
Autore:
SOARES IS; LEVITUS G; SOUZA JM; DELPORTILLO HA; RODRIGUES MM;
Indirizzi:
UNIV FED SAO PAULO,ESCOLA PAULISTA MED,DEPT MICROBIOL IMMUNOL & PARASITOL,RUA BOTUCATU 862 BR-04023062 SAO PAULO BRAZIL INST EVANDRO CHAGAS BR-66090000 BELEM PARA BRAZIL FED UNIV PARA,CTR CIENCIAS BIOL,DEPT PATOL BR-66075900 BELEM PARA BRAZIL UNIV SAO PAULO,DEPT PARASITOL BR-05508900 SAO PAULO BRAZIL
Titolo Testata:
Infection and immunity
fascicolo: 5, volume: 65, anno: 1997,
pagine: 1606 - 1614
SICI:
0019-9567(1997)65:5<1606:AITTNA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
INHIBIT PARASITE GROWTH; HUMAN-IGG RESPONSES; MONOCLONAL-ANTIBODIES; SERUM ANTIBODIES; RODENT MALARIA; AOTUS MONKEYS; T-CELLS; FALCIPARUM; FRAGMENT; ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
I.S. Soares et al., "ACQUIRED IMMUNE-RESPONSES TO THE N-TERMINAL AND C-TERMINAL REGIONS OFPLASMODIUM-VIVAX MEROZOITE SURFACE PROTEIN-1 IN INDIVIDUALS EXPOSED TO MALARIA", Infection and immunity, 65(5), 1997, pp. 1606-1614

Abstract

In this study, we evaluated the naturally acquired immune response toPlasmodium vivax merozoite surface protein 1 (PvMSP1) in individuals with recent clinical episodes of malaria from the state of Para, Brazil. Ten recombinant proteins representing the first 682 amino acids (aa) of the N-terminal region and one representing the final 111 aa of the C-terminal region were expressed in Escherichia coli as glutathione S-transferase fusion proteins. Both of these regions have been suggested as candidates for development of a vaccine against Plasmodium sp. The total frequencies of individuals with antibodies and cellular immune responses to PvMSP1 were high (83.8 and 75%, respectively). The recombinant proteins representing the N- and C-terminal regions were recognized by 51.4 and 64.1% of sera, respectively. The frequency of responders to the C-terminal region increased according to the number of previous malaria episodes, reaching 83.3% after four episodes. Cellular immune response was measured by in vitro proliferation and gamma interferon production. Peripheral blood mononuclear cells of 75 adn 47.2% ofindividuals proliferated in response to stimulation by the N- and C-terminal regions, respectively. Also, we found that one protein representing the N terminus and a second representing the C terminus of PvMSP1 stimulated 54.5% of individuals to secrete gamma interferon. We concluded that PvMSP1 is immunogenic to a large proportion of individuals exposed to malaria. Our results also suggested that the C-terminal region of PvMSP1 containing the two epidermal growth factor-like domains is particularly immunogenic to antibodies and T cells during natural infection in humans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 04:34:34