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Titolo:
CONFORMATIONAL CORRECTION MECHANISMS AIDING ANTIGEN RECOGNITION BY A HUMANIZED ANTIBODY
Autore:
HOLMES MA; BUSS TN; FOOTE J;
Indirizzi:
FRED HUTCHINSON CANC RES CTR,DIV MOL MED,1100 FAIRVIEW AVE N,C3-168,POB 19024 SEATTLE WA 98109 FRED HUTCHINSON CANC RES CTR,DIV MOL MED SEATTLE WA 98109
Titolo Testata:
The Journal of experimental medicine
fascicolo: 4, volume: 187, anno: 1998,
pagine: 479 - 485
SICI:
0022-1007(1998)187:4<479:CCMAAR>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESHAPING HUMAN-ANTIBODIES; HUMAN-IMMUNOGLOBULIN; ESCHERICHIA-COLI; WATER-MOLECULES; FRAGMENT; REGION; COMPLEMENTARITY; RESOLUTION; MOUSE; LOCUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
M.A. Holmes et al., "CONFORMATIONAL CORRECTION MECHANISMS AIDING ANTIGEN RECOGNITION BY A HUMANIZED ANTIBODY", The Journal of experimental medicine, 187(4), 1998, pp. 479-485

Abstract

The crystal structure of the complex between hen egg lysozyme and theFv fragment of a humanized antilysozyme antibody was determined to 2.7-Angstrom resolution. The structure of the antigen combining site in the complex is nearly identical to that of the complexed form of the parent mouse antibody, D1.3. In cont rast, the combining sites of the unliganded mouse and humanized antilysozymes show moderate conformational differences. This disparity suggests that a conformational readjustment process linked to antigen binding reverses adverse conformations in the complementarity determining regions that had been introduced byengineering these segments next to human framework regions in the humanized antibody.

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Documento generato il 25/09/20 alle ore 00:10:38