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Titolo:
A RAPID METHOD TO MONITOR REPAIR AND MIS-REPAIR OF DNA DOUBLE-STRAND BREAKS BY USING CELL-EXTRACTS OF THE YEAST SACCHAROMYCES-CEREVISIAE
Autore:
JHA B; AHNE F; KISTLER M; KLAUS C; ECKARDTSCHUPP F;
Indirizzi:
GSF FORSCHUNGSZENTRUM UMWELT & GESUNDHEIT GMBH,INST STRAHLENBIOL,POSTFACH 1129 D-85758 OBERSCHLEISSHEIM GERMANY GSF FORSCHUNGSZENTRUM UMWELT & GESUNDHEIT GMBH,INST STRAHLENBIOL D-85758 OBERSCHLEISSHEIM GERMANY LN MITHILA UNIV,DEPT BIOTECHNOL DARBHANGA 846008 INDIA
Titolo Testata:
Current genetics
fascicolo: 1, volume: 33, anno: 1998,
pagine: 1 - 3
SICI:
0172-8083(1998)33:1<1:ARMTMR>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONHOMOLOGOUS RECOMBINATION; ILLEGITIMATE RECOMBINATION; HOMOLOGOUS RECOMBINATION; NUCLEAR EXTRACT; DIVERGED DNAS; WILD-TYPE; END; TRANSFORMATION; MECHANISM; MUTATIONS;
Keywords:
YEAST CELLULAR EXTRACT; DNA DOUBLE-STRAND BREAK; FIDELITY OF REPAIR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
B. Jha et al., "A RAPID METHOD TO MONITOR REPAIR AND MIS-REPAIR OF DNA DOUBLE-STRAND BREAKS BY USING CELL-EXTRACTS OF THE YEAST SACCHAROMYCES-CEREVISIAE", Current genetics, 33(1), 1998, pp. 1-3

Abstract

We present a rapid in vitro method to scan the repair of DNA double-strand breaks (DSBs). A DSB was introduced at the EcoRI site within thelacZ gene of the plasmid pUC18 and the plasmid was exposed to cellular extracts from a wild-type repair-competent (RAD) and a mutant (rad52Delta) strain of the yeast Saccharomyces cerevisiae. The fidelity of rejoining was determined by the expression of the lacZ gene after bacterial transformation with the treated plasmid. A cellular extract fromthe yeast S. cerevisiae was found to be capable of rejoining DNA DSBs. Breaks at the EcoRI site were rejoined by extracts from both wild-type and mutant strains to form circular plasmids with almost equal efficiency. However, the fidelity of rejoining was lower for the rad52 Delta extract than for normal wild-type.

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Documento generato il 24/09/20 alle ore 05:21:46