Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ACTIVATION OF THE P42 MITOGEN-ACTIVATED PROTEIN-KINASE PATHWAY INHIBITS CDC2 ACTIVATION AND ENTRY INTO M-PHASE IN CYCLING XENOPUS EGG EXTRACTS
Autore:
BITANGCOL JC; CHAU ASS; STADNICK E; LOHKA MJ; DICKEN B; SHIBUYA EK;
Indirizzi:
UNIV ALBERTA,DEPT CELL BIOL & ANAT,MOL MECHANISMS GROWTH CONTROL GRP EDMONTON AB T6G 2H7 CANADA UNIV ALBERTA,DEPT CELL BIOL & ANAT,MOL MECHANISMS GROWTH CONTROL GRP EDMONTON AB T6G 2H7 CANADA UNIV CALGARY,DEPT BIOL SCI CALGARY AB T2N 1N4 CANADA
Titolo Testata:
Molecular biology of the cell
fascicolo: 2, volume: 9, anno: 1998,
pagine: 451 - 467
SICI:
1059-1524(1998)9:2<451:AOTPMP>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPINDLE-ASSEMBLY CHECKPOINT; MATURATION-PROMOTING FACTOR; SIGNAL-REGULATED KINASES; CELL-FREE SYSTEM; MAP KINASE; MEIOTIC MATURATION; METAPHASE ARREST; IN-VIVO; PHOSPHORYLATION; OOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
J.C. Bitangcol et al., "ACTIVATION OF THE P42 MITOGEN-ACTIVATED PROTEIN-KINASE PATHWAY INHIBITS CDC2 ACTIVATION AND ENTRY INTO M-PHASE IN CYCLING XENOPUS EGG EXTRACTS", Molecular biology of the cell, 9(2), 1998, pp. 451-467

Abstract

We have added constitutively active MAP kinase/ERK kinase (MEK), an activator of the mitogen-activated protein kinase (MAPK) signaling pathway, to cycling Xenopus egg extracts at various times during the cell cycle. p42MAPK activation during entry into M-phase arrested the cell cycle in metaphase, as has been shown previously. Unexpectedly, p42MAPK activation during interphase inhibited entry into M-phase. Ln these interphase-arrested extracts, H1 kinase activity remained low, Cdc2 was tyrosine phosphorylated, and nuclei continued to enlarge. The interphase arrest was overcome by recombinant cyclin B. Ln other experiments, p42MAPK activation by MEK or by Mos inhibited Cdc2 activation by cyclin B. PD098059, a specific inhibitor of MEK, blocked the effects of MEK(QP) and Mos. Mos-induced activation of P42MAPK did not inhibit DNA replication. These results indicate that, in addition to the established role of p42MAPK activation in M-phase arrest, the inappropriate activation of p42MAPK during interphase prevents normal entry into M-phase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 10:13:05