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Titolo:
14-3-3-PROTEIN ACT AS NEGATIVE REGULATORS OF THE INDUCER CDC25 IN XENOPUS EGG EXTRACTS
Autore:
KUMAGAI A; YAKOWEC PS; DUNPHY WG;
Indirizzi:
CALTECH,HOWARD HUGHES MED INST,DIV BIOL,216-76 PASADENA CA 91125 CALTECH,HOWARD HUGHES MED INST,DIV BIOL PASADENA CA 91125
Titolo Testata:
Molecular biology of the cell
fascicolo: 2, volume: 9, anno: 1998,
pagine: 345 - 354
SICI:
1059-1524(1998)9:2<345:1AANRO>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CYCLE REGULATION; DNA-REPLICATION; FISSION YEAST; SERINE KINASE; HUMAN WEE1; PHOSPHORYLATION; PHOSPHATASE; MITOSIS; CHECKPOINT; INITIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
A. Kumagai et al., "14-3-3-PROTEIN ACT AS NEGATIVE REGULATORS OF THE INDUCER CDC25 IN XENOPUS EGG EXTRACTS", Molecular biology of the cell, 9(2), 1998, pp. 345-354

Abstract

Cdc25, the dual-specificity phosphatase that dephosphorylates the Cdc2-cyclin B complex at mitosis, is highly regulated during the cell cycle. In Xenopus egg extracts, Cdc25 is associated with two isoforms of the 14-3-3 protein. Cdc25 is complexed primarily with 14-3-3 epsilon and to a lesser extent with 14-3-3 zeta. The association of these 14-3-3 proteins with Cdc25 varies dramatically during the cell cycle: binding is high during interphase but virtually absent at mitosis. Interaction with 14-3-3 is mediated by phosphorylation of Xenopus Cdc25 at Ser-287, which resides in a consensus 14-3-3 binding site. Recombinant Cdc25 with a point mutation at this residue (Cdc25-S287A) is incapable of binding to 14-3-3. Addition of the Cdc25-S287A mutant to Xenopus eggextracts accelerates mitosis and overrides checkpoint-mediated arrests of mitotic entry due to the presence of unreplicated and damaged DNA. These findings indicate that 14-3-3 proteins act as negative regulators of Cdc25 in controlling the G(2)-M transition.

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Documento generato il 26/09/20 alle ore 17:35:11