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Titolo:
AN IMAGE CORRELATION-ANALYSIS OF THE DISTRIBUTION OF CLATHRIN ASSOCIATED ADAPTER PROTEIN (AP-2) AT THE PLASMA-MEMBRANE
Autore:
BROWN CM; PETERSEN NO;
Indirizzi:
UNIV WESTERN ONTARIO,DEPT CHEM LONDON ON N6A 5B7 CANADA UNIV WESTERN ONTARIO,DEPT CHEM LONDON ON N6A 5B7 CANADA
Titolo Testata:
Journal of Cell Science
, volume: 111, anno: 1998,
parte:, 2
pagine: 271 - 281
SICI:
0021-9533(1998)111:<271:AICOTD>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE CORRELATION SPECTROSCOPY; COATED VESICLE ADAPTERS; GROWTH-FACTOR RECEPTORS; COMPLEX AP-2; HUMAN FIBROBLASTS; APPENDAGE DOMAIN; BINDING SUBUNIT; INVITRO BINDING; SORTING SIGNALS; ALPHA-ADAPTIN;
Keywords:
ADAPTER PROTEIN (AP-2); DISTRIBUTION; CLATHRIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
67
Recensione:
Indirizzi per estratti:
Citazione:
C.M. Brown e N.O. Petersen, "AN IMAGE CORRELATION-ANALYSIS OF THE DISTRIBUTION OF CLATHRIN ASSOCIATED ADAPTER PROTEIN (AP-2) AT THE PLASMA-MEMBRANE", Journal of Cell Science, 111, 1998, pp. 271-281

Abstract

Clathrin associated adaptor protein is involved in endocytosis at theplasma membrane (AP-2) and protein sorting at the Golgi membrane (AP-1), There is a great deal of information available on the structure, function and binding characteristics of AP-2, however, there is little quantitative data on the AP-2 distribution at the membrane, Image correlation spectroscopy is a technique which yields number counts from anautocorrelation analysis of intensity fluctuations within confocal microscopy images, Image correlation spectroscopy analysis of the indirect immunofluorescence from AP-2 at the plasma membrane of CV-1 cells shows that AP-2 is in a bimodal distribution consisting of large coatedpit associated aggregates of similar to 60 AP-2 molecules, and smaller aggregates containing similar to 20 AP-2 propose are coated pit nucleation hypertonic treatment 25 % of the AP-2 molecules dissociate molecules, which we sites, Following from the large AP-2 aggregates and form AP-2 dimers, leaving the remaining AP-2 as large aggregates with similar to 45 molecules, The smaller AP-2 aggregates completely dissociate forming AP-2 dimers, Dispersion of AP-2 with hypertonic treatment is not seen qualitatively because the number of large AP-2 aggregates is unchanged, the aggregates are just 25% smaller, Change in temperature from 37 degrees C to 4 degrees C has no affect on the number of AP-2aggregates or the AP-2 distribution between the two populations, These data and estimates of the coated pit size suggest that coated pits cover similar to 0.9% of the cell membrane, Combination of image correlation spectroscopy analysis and measurements of the CV-1 cell surface area show that there are similar to 6x10(5) AP-2 molecules per CV-1 cell with similar to 2x10(5) AP-2 molecules within coated pit structures.

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Documento generato il 19/01/20 alle ore 09:46:46