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Titolo:
IDENTIFICATION OF A STEROIDOGENIC NEUROHORMONE IN FEMALE MOSQUITOS
Autore:
BROWN MR; GRAF R; SWIDEREK KM; FENDLEY D; STRACKER TH; CHAMPAGNE DE; LEA AO;
Indirizzi:
UNIV GEORGIA,DEPT ENTOMOL ATHENS GA 30602 UNIV ZURICH HOSP,DEPT CHIRURG CH-8091 ZURICH SWITZERLAND CITY HOPE NATL MED CTR,DIV IMMUNOL DUARTE CA 91010
Titolo Testata:
The Journal of biological chemistry
fascicolo: 7, volume: 273, anno: 1998,
pagine: 3967 - 3971
SICI:
0021-9258(1998)273:7<3967:IOASNI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; AEDES-AEGYPTI; MICROSEQUENCE ANALYSIS; PEPTIDES; PROTEINS; VITELLOGENIN; HORMONE; SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
M.R. Brown et al., "IDENTIFICATION OF A STEROIDOGENIC NEUROHORMONE IN FEMALE MOSQUITOS", The Journal of biological chemistry, 273(7), 1998, pp. 3967-3971

Abstract

In the female mosquito, Aedes aegypti, neurohormones are released from the brain in response to a blood meal and stimulate the ovaries to secrete ecdysteroid hormones, which modulate yolk protein synthesis in the fat body. Neuropeptides with this bioactivity were isolated from head extracts, and partial sequences from these peptides when aligned gave a 31-residue sequence at the amino terminus. Oligonucleotide primers for this sequence were used to amplify with the polymerase chain reaction a genomic DNA product that hybridized to a clone from a head cDNA library. The cDNA encodes a 149-residue preprohormone that is processed into an 86-residue peptide, as indicated by the mass value obtained from the native peptide, with the expected amino-terminal sequence,After modification, the cDNA for the putative neurohormone was expressed in a bacterial system, and the purified peptide had high specific activity in bioassays, thus confirming that it is a steroidogenic gonadotropin, the first to be identified for invertebrates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 05:02:39