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Titolo:
PURIFICATION AND PROPERTIES OF RECOMBINANT BETA-GALACTOSIDASE FROM BACILLUS-CIRCULANS
Autore:
FUJIMOTO H; MIYASATO M; ITO Y; SASAKI T; AJISAKA K;
Indirizzi:
MEIJI INST HLTH SCI NARUDA ODAWARA 250 JAPAN MEIJI CENT RES INST NARUDA ODAWARA 250 JAPAN
Titolo Testata:
Glycoconjugate journal
fascicolo: 2, volume: 15, anno: 1998,
pagine: 155 - 160
SICI:
0282-0080(1998)15:2<155:PAPORB>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONVENIENT SYNTHESIS; ESCHERICHIA-COLI; SEQUENTIAL USE; BOVINE TESTES; CARBOHYDRATE; DERIVATIVES; XANTHOMONAS; ORIGINS;
Keywords:
BETA-GALACTOSIDASE; BACILLUS CIRCULANS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
H. Fujimoto et al., "PURIFICATION AND PROPERTIES OF RECOMBINANT BETA-GALACTOSIDASE FROM BACILLUS-CIRCULANS", Glycoconjugate journal, 15(2), 1998, pp. 155-160

Abstract

A gene encoding beta-galactosidase from Bacillus circulans which had hydrolysis specificity for the beta 1-3 linkage was expressed in Escherichia coli. The beta-galactosidase was purified from crude cell lysates of E. coli by column chromatographies on Resource Q and Sephacryl S-200 HR. The enzyme released galactose with high selectivity from oligosaccharides which had terminal beta 1-3 linked galactose residues. However it did not hydrolyse beta 1-4 linked galactooligosaccharides. Moreover, Gal beta 1-3GlcNAc, Gal beta 1-3GalNAc, and their p-nitrophenyl glycosides were regioselectively synthesized in 10-46% yield by the transglycosylation reaction using this enzyme.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 11:53:54