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Titolo:
DEVELOPMENT AND CHARACTERIZATION OF AN ANTIBODY-DIRECTED TO AN ALPHA-N-ACETYL-D-GALACTOSAMINE GLYCOSYLATED MUC2 PEPTIDE
Autore:
REIS CA; SORENSEN T; MANDEL U; DAVID L; MIRGORODSKAYA E; ROEPSTORFF P; KIHLBERG J; HANSEN JES; CLAUSEN H;
Indirizzi:
FAC HLTH SCI,SCH DENT,NORRE ALLE 20 DK-2200 COPENHAGEN DENMARK FAC HLTH SCI,SCH DENT DK-2200 COPENHAGEN DENMARK UNIV OPORTO,IPATIMUP P-4200 OPORTO PORTUGAL UNIV OPORTO,INST MOL PATHOL & IMMUNOL P-4200 OPORTO PORTUGAL ODENSE UNIV,DEPT MOL BIOL DK-5230 ODENSE DENMARK UMEA UNIV S-90187 UMEA SWEDEN HVIDOVRE UNIV HOSP,INFECT DIS LAB DK-2650 HVIDOVRE DENMARK
Titolo Testata:
Glycoconjugate journal
fascicolo: 1, volume: 15, anno: 1998,
pagine: 51 - 62
SICI:
0282-0080(1998)15:1<51:DACOAA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE SYNTHESIS; T-CELL RECOGNITION; MONOCLONAL-ANTIBODIES; BLOOD-GROUP; CARBOHYDRATE EPITOPES; IMMUNE-RESPONSE; CANCER PATIENTS; COLON CANCER; CDNA CLONING; ANTIGEN;
Keywords:
TN-ANTIGEN; MUC2; MUCIN; GLYCOPEPTIDE; CARBOHYDRATE ANTIGEN; O-GLYCOSYLATION; MONOCLONAL ANTIBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Reis et al., "DEVELOPMENT AND CHARACTERIZATION OF AN ANTIBODY-DIRECTED TO AN ALPHA-N-ACETYL-D-GALACTOSAMINE GLYCOSYLATED MUC2 PEPTIDE", Glycoconjugate journal, 15(1), 1998, pp. 51-62

Abstract

In an attempt to raise anti-Tn antibodies, an alpha-N-acetyl-D-galactosamine glycosylated peptide based on the tandem repeat of the intestinal mucin MUC2 was used as an immunogen. The MUC2 peptide (PTTTPISTTTMVTPTPTPTC) was glycosylated in vitro using concentrated alpha-N-acetylgalactosaminyltransferases activity from porcine submaxillary glands which resulted in the incorporation of 8-9 mol of Ga/NAc. Rabbits and mice developed specific anti-MUC2-GalNAc glycopeptide antibodies and nodetectable anti-Tn antibodies. Anti-glycopeptide antibodies did not show reactivity with the unglycosylated MUC2 peptide or with other GalNAc glycosylated peptides. A mouse monoclonal antibody (PMH1) representative of the observed immune response was generated and its immunohistological reactivity analysed in normal tissues. PMH1 reacted similarlyto other anti-MUC2 peptide antibodies. However, in some cells the staining was not restricted to the supranuclear area but extended to the entire cytoplasm. In addition, PMH1 reacted with purified colonic mucin by Western blot analysis suggesting that PMH1 reacted with some glycoforms of MUC2. The present work presents a useful approach for development of anti-mucin antibodies directed to different glycoforms of individual mucins.

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Documento generato il 24/11/20 alle ore 14:11:02