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Titolo:
FLUORESCENT VIRIONS - DYNAMIC TRACKING OF THE PATHWAY OF ADENOVIRAL GENE-TRANSFER VECTORS IN LIVING CELLS
Autore:
LEOPOLD PL; FERRIS B; GRINBERG I; WORGALL S; HACKETT NR; CRYSTAL RG;
Indirizzi:
CORNELL UNIV,NEW YORK HOSP,MED CTR,DIV PULM & CRIT CARE MED,520 E 70TH ST,ST505 NEW YORK NY 10021 CORNELL UNIV,NEW YORK HOSP,MED CTR,DIV PULM & CRIT CARE MED NEW YORK NY 10021
Titolo Testata:
Human gene therapy
fascicolo: 3, volume: 9, anno: 1998,
pagine: 367 - 378
SICI:
1043-0342(1998)9:3<367:FV-DTO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-MEDIATED ENDOCYTOSIS; PENTON BASE PROTEIN; HELA-CELLS; EARLY EVENTS; VIRUS ENTRY; INTERNALIZATION MOTIF; MICROTUBULES INVITRO; COOPERATIVE BINDING; DEPENDENT RELEASE; CYSTIC-FIBROSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
71
Recensione:
Indirizzi per estratti:
Citazione:
P.L. Leopold et al., "FLUORESCENT VIRIONS - DYNAMIC TRACKING OF THE PATHWAY OF ADENOVIRAL GENE-TRANSFER VECTORS IN LIVING CELLS", Human gene therapy, 9(3), 1998, pp. 367-378

Abstract

The pathogenic agent, adenovirus (Ad), has taken on a new role as a vector for gene transfer in both laboratory and clinical settings, To help understand the intracellular pathways and fate of Ad gene transfervectors, we covalently conjugated fluorophores to E1(-), E3(-) Ad vectors and used quantitative fluorescence microscopy to assess essentialsteps of Ad vector gene transfer to the A549 human epithelial lung cell line including binding, internalization, escape from endosomes, translocation to the nucleus, dissociation of capsids and gene expression, The data demonstrate that Ad internalizes with a at(1/2) 2.5 min, breaks out of endosomes early, likely prior to endosome-endosome fusion,exhibits sustained, intracellular velocities averaging 0.58 mu m/sec,and translocates to the nucleus with >80% of internalized fluorophoredemonstrating nuclear localization within 60 min of infection, Interestingly, 24 hr after infection, half of the initially internalized fluorescence was detected but lacked nuclear localization, suggesting that the capsid is released from the nucleus and is likely degraded, Fluorescent labeling of virions provides a novel quantitative, morphological strategy to characterize the interaction of gene transfer vectors with the intracellular environment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 14:16:37