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Titolo:
SERUM DEPRIVATION AND PROTEIN-SYNTHESIS INHIBITION INDUCE 2 DIFFERENTAPOPTOTIC PROCESSES IN N18 NEUROBLASTOMA-CELLS
Autore:
BOIX J; FIBLA J; YUSTE VJ; PIULATS JM; LLECHA N; COMELLA JX;
Indirizzi:
UNIV LLEIDA,DEPT CIENCIES MED BASIQUES,MOL NEUROBIOL GRP,AV ROVIRA ROURE 44 LLEIDA 25198 CATALONIA SPAIN UNIV LLEIDA,DEPT CIENCIES MED BASIQUES,MOL NEUROBIOL GRP LLEIDA 25198CATALONIA SPAIN
Titolo Testata:
Experimental cell research
fascicolo: 2, volume: 238, anno: 1998,
pagine: 422 - 429
SICI:
0014-4827(1998)238:2<422:SDAPII>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
BCL-2 HOMOLOG BAK; C-MYC; ENDOPLASMIC-RETICULUM; REGULATED APOPTOSIS; NUCLEAR-ENVELOPE; DEATH; SURVIVAL; EXECUTION; FAMILY; LINES;
Keywords:
APOPTOSIS; NEUROBLASTOMA; CYCLOHEXIMIDE; PROTEASE INHIBITORS; BCL-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
J. Boix et al., "SERUM DEPRIVATION AND PROTEIN-SYNTHESIS INHIBITION INDUCE 2 DIFFERENTAPOPTOTIC PROCESSES IN N18 NEUROBLASTOMA-CELLS", Experimental cell research, 238(2), 1998, pp. 422-429

Abstract

N18 are murine neuroblastoma cells that underwent cell death upon serum deprivation or inhibition of protein synthesis by means of cycloheximide (CHX), In both cases, an ultrastructural morphology and an internucleosomal pattern of DNA fragmentation typical of apoptosis were found. However, electron microscopy revealed abundant lipid vesicles in the cytoplasm of CHX-treated cells that were not found in their serum-deprived counterparts. In addition, when both types of apoptotic cells were compared by means of flow cytometry and chromatin staining with propidium iodide, the former showed consistently less fluorescence thanthe latter. Therefore, in N18 cells, both apoptotic processes seemed to differ at a structural level. At a functional level, we found that apoptosis was blocked by the protease inhibitor TLCK in CHX-treated but not in serum-deprived cells. On the other hand, we generated N18 clones that overexpressed Bcl-2 protein. After a period of 48 h we found that identical levels of Bcl-2 protein were able to block apoptosis inserum-deprived but not in CHX-treated cells, In conclusion, two different biochemical pathways leading to apoptosis seem to coexist in N18 neuroblastoma cells, (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 21:37:39