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Titolo:
ANTISENSE BASIC FIBROBLAST GROWTH-FACTOR GENE-TRANSFER REDUCES EARLY INTIMAL THICKENING IN A RABBIT FEMORAL-ARTERY BALLOON INJURY MODEL
Autore:
NESCHIS DG; SAFFORD SD; HANNA AK; FOX JC; GOLDEN MA;
Indirizzi:
HOSP UNIV PENN,DEPT SURG,4 SILVERSTEIN PAVIL,3400 SPRUCE ST PHILADELPHIA PA 19104 UNIV PENN,MED CTR,DEPT MED PHILADELPHIA PA 19104 UNIV PENN,MED CTR,DEPT SURG PHILADELPHIA PA 19104
Titolo Testata:
Journal of vascular surgery
fascicolo: 1, volume: 27, anno: 1998,
pagine: 126 - 134
SICI:
0741-5214(1998)27:1<126:ABFGGR>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; VASCULAR INJURY; DIRECTIONAL ATHERECTOMY; CORONARY-ARTERY; LESIONS; PROLIFERATION; ANGIOPLASTY; HYPERPLASIA; CILAZAPRIL; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
D.G. Neschis et al., "ANTISENSE BASIC FIBROBLAST GROWTH-FACTOR GENE-TRANSFER REDUCES EARLY INTIMAL THICKENING IN A RABBIT FEMORAL-ARTERY BALLOON INJURY MODEL", Journal of vascular surgery, 27(1), 1998, pp. 126-134

Abstract

Purpose: The purpose of this study was to determine whether endogenous basic fibroblast growth factor (bFGF) production contributes to the intimal hyperplastic response to injury in a model of rabbit femoral artery balloon injury. Inhibition of de novo production of bFGF proteinwas targeted by intramural adenoviral gene transfer of antisense bFGF(Ad.ASbFGF) RNA. The adenovirus was delivered locally intraluminally at the time of arterial injury. Methods: New Zealand White rabbits underwent balloon injury of the superficial femoral artery, followed by intraluminal delivery of an adenoviral vector encoding a rat antisense bFGF (ASbFGF) transcript at a concentration of 1 X 10(10) plaque-forming units per milliliter. Control animals were treated in a similar fashion, using either an adenovirus encoding the lac reporter gene (Ad.lacZ) or phosphate-buffered saline solution (PBS; vehicle) alone. Two weeks after balloon injury, rabbits were killed and perfusion fixed. Femoral arteries were harvested for histomorphometric analysis. Intimal and medial wall thickness was measured at eight points around the vessel perimeter, and mean intimal/medial (I/M) thickness ratios were compared by analysis of variance and Student's t test. In addition, medial cell proliferation in Ad.ASbFGF and Ad.lacZ treated arteries was evaluated 4 days and 2 weeks after balloon injury by 5-Bromo-2'-deoxyuridine labeling. Results: At 14 days (n = 25) after balloon injury, histomorphometric analysis revealed a significant inhibition of intimal thickening in Ad.ASbFGF-treated arteries as compared with Ad.lacZ-treated and PBS-treated controls (I/M thickness ratios +/- SD, 0.43 +/- 0.22 for Ad.ASbFGF vs 1.03 +/- 0.28 for Ad.lacZ and 0.86 +/- 0.19 for PBS; p < 0.0001 and p = 0.004, respectively). There was no significant difference in the I/M thickness ratios of Ad.lacZ-treated and IFS-treated vessels (P = 0.27). Although there was no significant difference in the proliferation index of Ad.ASbFGF-treated and Ad.lacZ-treated vessels 4days after injury, an increase in apoptosis was noted in the Ad.ASbFGF-treated vessels 4 days after balloon injury. Conclusions: The use ofASbFGF RNA gene transfer, designed to inhibit de novo bFGF synthesis after balloon injury, results in a significant inhibition of neointimal formation. This suggests that continued bFGF synthesis contributes to the arterial response to injury in rabbits. ASbFGF gene transfer maybe an effective strategy in limiting the intimal hyperplastic response after vascular reconstructive procedures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 11:32:02