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Titolo:
CHARACTERIZATION OF AN ADENOVIRUS VECTOR CONTAINING HETEROLOGOUS PEPTIDE EPITOPE IN THE HI LOOP OF THE FIBER KNOB
Autore:
KRASNYKH V; DMITRIEV I; MIKHEEVA G; MILLER CR; BELOUSOVA N; CURIEL DT;
Indirizzi:
UNIV ALABAMA,CTR COMPREHENS CANC,GENE THERAPY PROGRAM,1824 6TH AVE S,ROOM WTI 620 BIRMINGHAM AL 35294 UNIV ALABAMA,CTR COMPREHENS CANC,GENE THERAPY PROGRAM BIRMINGHAM AL 35294
Titolo Testata:
Journal of virology
fascicolo: 3, volume: 72, anno: 1998,
pagine: 1844 - 1852
SICI:
0022-538X(1998)72:3<1844:COAAVC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONKEY AIRWAY EPITHELIUM; MEDIATED GENE-TRANSFER; HUMAN CFTR CDNA; RECOMBINANT ADENOVIRUS; ESCHERICHIA-COLI; BINDING DOMAIN; PENTON BASE; PROTEIN; RECEPTOR; THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
V. Krasnykh et al., "CHARACTERIZATION OF AN ADENOVIRUS VECTOR CONTAINING HETEROLOGOUS PEPTIDE EPITOPE IN THE HI LOOP OF THE FIBER KNOB", Journal of virology, 72(3), 1998, pp. 1844-1852

Abstract

The utility of the present generation of recombinant adenovirus vectors for gene therapy applications could potentially be improved by designing targeted vectors capable of gene delivery to selected cell typesin vivo. In order to achieve such targeting, we are investigating thepossibilities of incorporation of ligands in the adenovirus fiber protein, which mediates primary binding of adenovirus to its cell surfacereceptor. Based on the proposed structure of the cell-binding domain of the fiber, we hypothesized that the HI loop of the fiber knob can be utilized as a convenient locale for incorporation of heterologous ligands. In this study, He utilized recombinant fiber proteins expressedin baculovirus-infected insect cells to demonstrate that the incorporation of the FLAG octapeptide into the HI loop does not ablate fiber trimerization and does not disturb formation of the cell-binding site localized in the knob, We then generated a recombinant adenovirus containing this modified fiber and showed that the short peptide sequence engineered in the knob is compatible with the biological functions of the fiber, In addition, by using a ligand-specific antibody, we have shown that the peptide incorporated into the knob remains available for binding in the contest of mature virions containing modified fibers, These findings suggest that heterologous ligands can be incorporated into the HI loop of the fiber knob and that this locale possesses propelties consistent with its employment in adenovirus retargeting strategies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 07:26:37