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Titolo:
CDNA CLONING, SUBSTRATE-SPECIFICITY AND EXPRESSION STUDY OF TOBACCO CAFFEOYL-COA 3-O-METHYLTRANSFERASE, A LIGNIN BIOSYNTHETIC ENZYME
Autore:
MARTZ F; MAURY S; PINCON G; LEGRAND M;
Indirizzi:
CNRS,INST BIOL MOL PLANTES,12 RUE GEN ZIMMER F-67084 STRASBOURG FRANCE CNRS,INST BIOL MOL PLANTES F-67084 STRASBOURG FRANCE
Titolo Testata:
Plant molecular biology
fascicolo: 3, volume: 36, anno: 1998,
pagine: 427 - 437
SICI:
0167-4412(1998)36:3<427:CCSAES>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
COENZYME-A 3-O-METHYLTRANSFERASE; DIPHENOL-O-METHYLTRANSFERASES; CELL-SUSPENSION CULTURES; ADENOSYL-L-METHIONINE; MOSAIC-VIRUS; HYPERSENSITIVE REACTION; DISEASE RESISTANCE; MOLECULAR-CLONING; ELICITOR; PURIFICATION;
Keywords:
COENZYME A ESTERS; LIGNIN BIOSYNTHESIS; O-METHYLTRANSFERASE; PHENYLPROPANOIDS; TOBACCO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
F. Martz et al., "CDNA CLONING, SUBSTRATE-SPECIFICITY AND EXPRESSION STUDY OF TOBACCO CAFFEOYL-COA 3-O-METHYLTRANSFERASE, A LIGNIN BIOSYNTHETIC ENZYME", Plant molecular biology, 36(3), 1998, pp. 427-437

Abstract

Four caffeoyl-CoA 3-O-methyltransferase (CCoAOMT) cDNA clones were isolated from RNA extracted from TMV-infected tobacco leaves using an heterologous DNA probe. The cDNAs were 84-93% identical in their nucleotide sequences, indicating that they are the products of four closely related genes. A comparison of the CCoAOMT cDNAs with database sequences and Southern blot analysis indicated that they are encoded by a new CCoAOMT family of tobacco. Overall expression of this gene family in tobacco tissues was investigated by RNA blot analysis. The expression of each individual gene was studied by RT-PCR coupled with RFLP analysis of PCR products, taking advantage of the presence of specific restriction sites in each cloned cDNA. Two members of the CCoAOMT gene family appeared to be constitutively expressed in various plant organs and tissues whereas the two others were preferentially expressed in flowerorgans, after tobacco mosaic virus (TMV) infection or elicitor treatment of leaves. The CCoAOMT enzymatic protein expressed in bacteria waspurified and shown to be specific for the caffeoyl-CoA and 5-hydroxyferuloyl-CoA esters and to have no activity against free caffeic acid and 5-hydroxyferulic acid. The pattern of CCoAOMT transcript accumulation during development of tobacco stem was found closely related to that of COMT I genes which have been shown to be specifically involved inlignin biosynthesis. Moreover, the inhibition of COMT I gene expression in transgenic tobacco was also shown to decrease CCoAOMT gene expression, particularly in the most lignified tissues. Thus, the expression pattern and the substrate specificity of tobacco CCoAOMT sustain a preferential role in lignin biosynthesis.

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Documento generato il 02/04/20 alle ore 18:07:51