Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
GROWTH-HORMONE RECEPTOR EXPRESSION IN THE NUCLEUS AND CYTOPLASM OF NORMAL AND NEOPLASTIC-CELLS
Autore:
LINCOLN DT; SINOWATZ F; TEMMIMBAKER L; BAKER HI; KOLLE S; WATERS MJ;
Indirizzi:
KUWAIT UNIV,FAC ALLIED HLTH,DEPT MED SCI,POB 31470 SULIBIKHAT 90805 KUWAIT UNIV MUNICH,INST VET ANAT D-80539 MUNICH GERMANY KUWAIT CANC CONTROL CTR KUWAIT KUWAIT UNIV QUEENSLAND,DEPT PHYSIOL & PHARMACOL ST LUCIA QLD 4067 AUSTRALIA
Titolo Testata:
HISTOCHEMISTRY AND CELL BIOLOGY
fascicolo: 2, volume: 109, anno: 1998,
pagine: 141 - 159
SICI:
0948-6143(1998)109:2<141:GREITN>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-BREAST-CANCER; MESSENGER RIBONUCLEIC-ACIDS; SERUM BINDING-PROTEIN; I GENE-EXPRESSION; MAMMARY-GLAND; INSULIN; RAT; GH; IDENTIFICATION; PURIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
79
Recensione:
Indirizzi per estratti:
Citazione:
D.T. Lincoln et al., "GROWTH-HORMONE RECEPTOR EXPRESSION IN THE NUCLEUS AND CYTOPLASM OF NORMAL AND NEOPLASTIC-CELLS", HISTOCHEM C, 109(2), 1998, pp. 141-159

Abstract

Growth hormone (GH) exerts its regulatory functions in controlling metabolism, balanced growth and differentiated cell expression by actingon specific receptors which trigger a phosphorylation cascade, resulting in the modulation of numerous signalling pathways dictating gene expression. A panel of five monoclonal antibodies was used in mapping the presence and somatic distribution of the GH receptor by immunohistochemistry in normal and neoplastic tissues and cultured cells of human, rat and rabbit origin. A wide distribution of the receptor was observed in many cell types. Not all cells expressing cytoplasmic GH receptors displayed nuclear immunoreactivity. In general, the relative proportion of positive cells and intensity of staining was higher in neoplastic cells than in normal tissue cells. Immunoreactivity showed subcellular localisation of the GH receptor in cell membranes and was predominantly cytoplasmic, but strong nuclear immunoreaction was also apparent in many instances. Intense immunoreactivity was also observed in the cellular Golgi area of established cell lines and cultured tissue-derived cells in exponential growth phase, indicating cells are capable of GH receptor synthesis. The presence of intracellular GH receptor, previously documented in normal tissues of mostly animal origin, is theresult of endoplasmic reticulum and Golgi localisation. Heterogeneityof immunoreactivity was found in normal and neoplastic tissue with a variable range of positive cells. The nuclear localisation of immunoreactivity is the result of nuclear GH receptor/binding protein, identically to the cytosolic and plasma GH-binding protein, using a panel of five monoclonal antibodies against the GH receptor extracellular region. The expression of GH receptors, not only on small proliferating tumour cells such as lymphocytes, but also on well differentiated cells including keratinocytes, suggests that GH is necessary not only for differentiation of progenitor cells, but also for their subsequent clonalexpansion, differentiation and maintenance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 10:00:15