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Titolo:
THE SEQUENCE AND STRUCTURE OF THE 3'-UNTRANSLATED REGIONS OF CHLOROPLAST TRANSCRIPTS ARE IMPORTANT DETERMINANTS OF MESSENGER-RNA ACCUMULATION AND STABILITY
Autore:
ROTT R; LIVEANU V; DRAGER RG; STERN DB; SCHUSTER G;
Indirizzi:
TECHNION ISRAEL INST TECHNOL,DEPT BIOL IL-32000 HAIFA ISRAEL TECHNION ISRAEL INST TECHNOL,DEPT BIOL IL-32000 HAIFA ISRAEL CORNELL UNIV,BOYCE THOMPSON INST PLANT RES ITHACA NY 14853
Titolo Testata:
Plant molecular biology
fascicolo: 2, volume: 36, anno: 1998,
pagine: 307 - 314
SICI:
0167-4412(1998)36:2<307:TSASOT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
3' INVERTED REPEATS; MESSENGER-RNA; CHLAMYDOMONAS-REINHARDTII; SPINACH CHLOROPLAST; END MATURATION; ATPB GENE; PROTEIN; BINDING; EXPRESSION; TURNOVER;
Keywords:
CHLAMYDOMONAS REINHARDTII; CHLOROPLAST; INVERTED REPEAT; 3'-UNTRANSLATED REGION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
R. Rott et al., "THE SEQUENCE AND STRUCTURE OF THE 3'-UNTRANSLATED REGIONS OF CHLOROPLAST TRANSCRIPTS ARE IMPORTANT DETERMINANTS OF MESSENGER-RNA ACCUMULATION AND STABILITY", Plant molecular biology, 36(2), 1998, pp. 307-314

Abstract

A general characteristic of the S'-untranslated regions (3' UTRs) of plastid mRNAs is an inverted repeat (IR) sequence that can fold into astem-loop structure. These stem-loops are RNA 3'-end processing signals and determinants of mRNA stability, not transcription terminators. Incubation of synthetic RNAs corresponding to the 3' UTRs of Chlamydomonas chloroplast genes atpB and petD with a chloroplast protein extract resulted in the accumulation of stable processing products. Synthetic RNAs of the petA 3' UTR and the antisense strand of atpB 3' UTR weredegraded in the extract. To examine 3' UTR function in vivo, the atpB3' UTR was replaced with the 3' UTR sequences of the Chlamydomonas chloroplast genes petD, petD plus trnR, rbcL, petA and E. coli thrA by biolistic transformation of Chlamydomonas chloroplasts. Each 3' UTR wasinserted in both the sense and antisense orientations. The accumulation of both total atpB mRNA and ATPase beta-subunit protein in all transformants was increased compared to a strain in which the atpB 3' UTR had been deleted. However, the level of discrete atpB transcripts in transformants containing the antisense 3' UTR sequences was reduced to approximately one-half that of transformants containing the 3' UTRs inthe sense orientation. These results imply that both the nucleotide sequences and the stem-loop structures of the 3' UTRs are important fortranscript 3'-end processing, and for accumulation of the mature mRNAs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 18:43:46