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Titolo:
ISOLATION AND PARTIAL CHARACTERIZATION OF A COTTON ROOT LIPASE
Autore:
PRIOLO NS; ARRIBERE MC; CAFFINI NO;
Indirizzi:
NATL UNIV LA PLATA,FAC CIENCIAS EXACTAS,DEPT CIENCIAS BIOL,LIPROVE,CASILLA CORREO 711 RA-1900 LA PLATA ARGENTINA
Titolo Testata:
Acta alimentaria
fascicolo: 1, volume: 26, anno: 1997,
pagine: 23 - 33
SICI:
0139-3006(1997)26:1<23:IAPCOA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PURIFICATION; OIL; HPLC;
Keywords:
LIPASE; COTTON; GOSSYPIUM HIRSUTUM;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
N.S. Priolo et al., "ISOLATION AND PARTIAL CHARACTERIZATION OF A COTTON ROOT LIPASE", Acta alimentaria, 26(1), 1997, pp. 23-33

Abstract

Crude extracts of cotton seedling roots (Gossypium hirsutum L. cv. Guazuncho2) exhibit lipolytic activity on cottonseed oil. When the crudeextract is centrifuged for 30 min at 4 degrees C and 11 000 g lipolytic activity is recovered from the pellet by adding 20 mmol Tris-HCl buffer (pH 8.5) containing 1% Triton X-100, 1 mmol DTT and 0.5 mmol EDTA. After centrifugation for 30 min at 50 000 g and for 1 h at 100.000 gthe enzyme is recovered in the supernatant. The lipase shows maximum activity at pH 8.0-8.5 and at 55 degrees C-65 degrees C and is stimulated by calcium. HPLC analysis of the remaining triacylglycerols (TAG) after the action of the lipase on cottonseed oil reveals higher affinity for TAG containing oleic acid. Fractionation of the soluble enzyme preparation by gel chromatography (Sephacryl S-300) shows the presenceof two active fractions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 09:28:11