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Titolo:
CHARACTERIZATION AND GENE SEQUENCING OF A 19-KDA PERIPLASMIC PROTEIN OF CAMPYLOBACTER-JEJUNI CAMPYLOBACTER-COLI/
Autore:
JANVIER B; CONSTANTINIDOU C; AUCHER P; MARSHALL ZV; PENN CW; FAUCHERE JL;
Indirizzi:
CHU LA MILETRIE,BP 577 F-86021 POITIERS FRANCE FAC MED & PHARM POITIERS FRANCE UNIV BIRMINGHAM,SCH BIOL SCI BIRMINGHAM B15 2TT W MIDLANDS ENGLAND
Titolo Testata:
Research in microbiology
fascicolo: 2, volume: 149, anno: 1998,
pagine: 95 - 107
SICI:
0923-2508(1998)149:2<95:CAGSOA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
OUTER-MEMBRANE PROTEINS; POLYMORPHISM ANALYSIS; TREPONEMA-PALLIDUM; ANTIBODY; ELECTROPHORESIS; IDENTIFICATION; ENTERITIS; ANTIGENS; CELLS; COLI;
Keywords:
ENTERITIS; CAMPYLOBACTER JEJUNI; CAMPYLOBACTER COLI; CELL WALL, PERIPLASMIC PROTEIN P19; GENE SEQUENCING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
B. Janvier et al., "CHARACTERIZATION AND GENE SEQUENCING OF A 19-KDA PERIPLASMIC PROTEIN OF CAMPYLOBACTER-JEJUNI CAMPYLOBACTER-COLI/", Research in microbiology, 149(2), 1998, pp. 95-107

Abstract

In order to study a 19-kDa protein (p19) of Campylobacter jejuni, we purified this protein to homogeneity from C. jejuni strain 81176 by anion exchange chromatography. The molecular weight of the native protein is 19,000 daltons. P19 was found to be acidic with an isoelectric point of 4.8 and was located in the periplasmic space of the bacteria. The 20 N-terminal amino acids were sequenced and no significant similarities with known proteins were shown. A monoclonal antibody showed that p19 is conserved in the 2 species C. jejuni and C. coli. Analysis ofsera from 23 patients with a Campylobacter-related infection indicated that pig is not immunogenic during natural infection in man. The gene encoding p19 was cloned and no strong homologies with known sequences were identified. The preparation of a knockout mutant in p19 will enable the investigation of the function of this cell wall component of Campylobacter.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 01:40:41