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Titolo:
PROTEIN-TYROSINE PHOSPHORYLATION BY IGG1 SUBCLASS CD38 MONOCLONAL-ANTIBODIES IS MEDIATED THROUGH STIMULATION OF THE FC-GAMMA-II RECEPTORS IN HUMAN MYELOID CELL-LINES
Autore:
INOUE S; KONTANI K; TSUJIMOTO N; KANDA Y; HOSODA N; HOSHINO S; HAZEKI O; KATADA T;
Indirizzi:
UNIV TOKYO,GRAD SCH PHARMACEUT SCI,DEPT PHYSIOL CHEM TOKYO 113 JAPAN UNIV TOKYO,GRAD SCH PHARMACEUT SCI,DEPT PHYSIOL CHEM TOKYO 113 JAPAN
Titolo Testata:
The Journal of immunology
fascicolo: 11, volume: 159, anno: 1997,
pagine: 5226 - 5232
SICI:
0022-1767(1997)159:11<5226:PPBISC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIC ADP-RIBOSE; SURFACE ANTIGEN CD38; C-CBL PROTOONCOGENE; PHOSPHATIDYLINOSITOL 3-KINASE; SIGNAL-TRANSDUCTION; NAD GLYCOHYDROLASE; MOLECULAR-CLONING; HUMAN-MONOCYTES; HL-60 CELLS; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
S. Inoue et al., "PROTEIN-TYROSINE PHOSPHORYLATION BY IGG1 SUBCLASS CD38 MONOCLONAL-ANTIBODIES IS MEDIATED THROUGH STIMULATION OF THE FC-GAMMA-II RECEPTORS IN HUMAN MYELOID CELL-LINES", The Journal of immunology, 159(11), 1997, pp. 5226-5232

Abstract

The human surface Ag CD38 is a 46-kDa type II transmembrane glycoprotetin, and its expression is dependent on the cell differentiation and activation of lymphocytes. Our previous work in human myeloid cells skewed that ligation of CD38 with mAbs (HB-7 and T-16; IgG1 subclass) not only induced protein-tryrosine phosphorylation but also potentiated superoxide generation stimulated by G protein-coupled receptors. In the present study we analyzed the mechanisms of action of the agonistic mAbs. HB-7-induced tyrosine phosphorylation could be still observed inhuman myeloid cells expressing CD38 mutants, of which cytoplasmic andtransmembrane domains had been deleted or replaced by those of another type II glycoprotein (PC-1). Moreover, N-linked glycosylation on thecell surface CD38 was not required for the HB-7-induced cell signaling. The profile of tyrosine-phosphorylated proteins by HB-7 was exactlythe same as that induced by cross-linking of Fc gamma II receptors (Fc gamma RII/CD32); and Fc gamma RII itself was tyrosine phosphorylatedin the two stimulated cells. The HB-7-inducd tyrosine phosphorylationwas completely abolished after masking of Fc gamma RII with its mAb. Finally, F(ab')(2) of HB-7 failed to mimic the actions of the whole form of mAb. These results indicate that anti-CD38 mAb-induced tyrosine phosphorylation and its associated cell response are entirely mediatedthrough the Fc gamma RII-induced signaling pathway, possibly resulting from stimulation of the cell surface human Fc gamma RII with the mouse Fc region (IgG1 subclass) of CD38-ligated mAbs.

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Documento generato il 30/11/20 alle ore 16:09:43