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Titolo:
AN INTRONIC SILENCER REGULATES B-LYMPHOCYTE CELL-SPECIFIC AND STAGE-SPECIFIC EXPRESSION OF THE HUMAN-COMPLEMENT RECEPTOR-TYPE 2 (CR-2, CD21) GENE
Autore:
MAKAR KW; PHAM CTN; DEHOFF MH; OCONNOR SM; JACOBI SM; HOLERS VM;
Indirizzi:
UNIV COLORADO,HLTH SCI CTR,DEPT MED,DIV RHEUMATOL,BOX B-115,4200 E 9TH AVE DENVER CO 80262 UNIV COLORADO,HLTH SCI CTR,DEPT MED,DIV RHEUMATOL DENVER CO 80262 UNIV COLORADO,HLTH SCI CTR,DEPT IMMUNOL,DIV RHEUMATOL DENVER CO 80262 WASHINGTON UNIV,SCH MED,DEPT MED,DIV RHEUMATOL ST LOUIS MO 63110
Titolo Testata:
The Journal of immunology
fascicolo: 3, volume: 160, anno: 1998,
pagine: 1268 - 1278
SICI:
0022-1767(1998)160:3<1268:AISRBC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPSTEIN-BARR-VIRUS; MEMBRANE COFACTOR PROTEIN; T-DEPENDENT ANTIGEN; MONOCLONAL-ANTIBODIES; C3D RECEPTOR; HUMAN CR-2; ATTACHMENT REGIONS; TRANSGENIC MICE; NUCLEAR MATRIX; IGE PRODUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
80
Recensione:
Indirizzi per estratti:
Citazione:
K.W. Makar et al., "AN INTRONIC SILENCER REGULATES B-LYMPHOCYTE CELL-SPECIFIC AND STAGE-SPECIFIC EXPRESSION OF THE HUMAN-COMPLEMENT RECEPTOR-TYPE 2 (CR-2, CD21) GENE", The Journal of immunology, 160(3), 1998, pp. 1268-1278

Abstract

Human CR2 (CD21) is a B lymphocyte protein whose surface expression is restricted primarily to the mature cell stage during development, Tostudy the transcriptional mechanisms that govern cell-and stage-restricted CR2 expression, we first performed transient transfection analysis using constructs extending from -5 kb to +75 bp (-5 kb/+75) in the CR2 promoter, The promoter was found to he broadly active, with no evidence of cell-or stage-specific reporter gene expression, However, theaddition of a 2,5-kb intronic gene segment (containing a DNase I hypersensitive site) to the (-5-kh/+75) construct resulted in appropriate reporter gene expression, defined as the silencing of the (-5-kb/+75) promoter activity only in non-CR2-expressing cells, Interestingly, appropriate reporter gene expression required stable transfection of the constructs in cell lines, suggesting nuclear matrix or chromatin interactions may be important for appropriate CR2 gene expression, Importantly, transgenic mice also required the intronic silencer to generate lymphoid tissue-specific reporter gene expression, Some transgenic founder lines did net demonstrate reporter gene expression, however, indicating that additional transcriptional regulatory elements are present in other regions of the CR2 gene, In summary, these data support the hypothesis that human CR2 expression is regulated primarily by an intronic silencer with lineage-and B cell stage-specific activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:26:02