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Titolo:
APICAL SECRETION OF A PATHOGEN-ELICITED EPITHELIAL CHEMOATTRACTANT ACTIVITY IN RESPONSE TO SURFACE COLONIZATION OF INTESTINAL EPITHELIA BY SALMONELLA-TYPHIMURIUM
Autore:
MCCORMICK BA; PARKOS CA; COLGAN SP; CARNES DK; MADARA JL;
Indirizzi:
MASSACHUSETTS GEN HOSP,DEPT PEDIAT GASTROENTEROL & NUTR,149 13TH ST 1493404 CHARLESTOWN MA 02129 HARVARD UNIV,SCH MED,BRIGHAM & WOMENS HOSP,DIV GASTROINTESTINAL PATHOL BOSTON MA 02115 HARVARD UNIV,SCH MED,BRIGHAM & WOMENS HOSP,DEPT PATHOL BOSTON MA 02115 HARVARD UNIV,SCH MED,BRIGHAM & WOMENS HOSP,CTR EXPT THERAPEUT & REPERFUS INJURY BOSTON MA 02115 HARVARD DIGEST DIS CTR BOSTON MA 02115
Titolo Testata:
The Journal of immunology
fascicolo: 1, volume: 160, anno: 1998,
pagine: 455 - 466
SICI:
0022-1767(1998)160:1<455:ASOAPE>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-NEUTROPHILS; POLYMORPHONUCLEAR LEUKOCYTES; CHEMOTACTIC FACTORS; URINARY-TRACT; CELLS; MIGRATION; MONOLAYERS; COLITIS; INTERLEUKIN-8; INFLAMMATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
B.A. Mccormick et al., "APICAL SECRETION OF A PATHOGEN-ELICITED EPITHELIAL CHEMOATTRACTANT ACTIVITY IN RESPONSE TO SURFACE COLONIZATION OF INTESTINAL EPITHELIA BY SALMONELLA-TYPHIMURIUM", The Journal of immunology, 160(1), 1998, pp. 455-466

Abstract

Modeling Salmonella-epithelial cell interaction in vitro has led to the realization that epithelial cells are crucial in orchestrating neutrophil (PMN) responses, in part by stimulating basolateral release of epithelial chemokines, including IL-8, However, such basolaterally released chemokines, while likely important in orchestration of PMN movement across the subepithelial matrix, are unlikely to be responsible for the final step of transepithelial migration of PMN and entry into the apical compartment. We now show that S. typhimurium attachment to T84 cell apical epithelial membranes induces polarized apical secretion of a pathogen-elicited epithelial chemoattractant (PEEC) bioactivity, Experiments employing semipurified PEEC indicate that it is released in a polarized apical fashion and is sufficient to explain the observedfinal step of transepithelial migration of PMN induced by Salmonella-apical membrane interaction, By preliminary physical characterization and profiles of PMN activation, PEEC appears to be a novel PMN chemotactic bioactivity, This 1- to 3-kDa nominal molecular mass chemokine-like bioactivity directly stimulates PMN via a pertussis toxin-sensitivereceptor and elicits a Ca2+ signal, While these latter features are shared by most other chemokines, analysis of PEEC-elicited PMN activation reveals that, unlike these other agonists, PEEC, even at saturatingconcentrations, elicits chemotactic activity in the absence of stimulation of superoxide production and/or release of primary and/or secondary granules. These data suggest that the apically released PEEC activity appears to represent a novel epithelial-derived chemoattractant that directs PMN movement across epithelial monolayers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 15:13:22