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Titolo:
POTENT INDUCTION OF A NEUTROPHIL AND EOSINOPHIL-RICH INFILTRATE IN-VIVO BY HUMAN MAST-CELL TRYPTASE - SELECTIVE ENHANCEMENT OF EOSINOPHIL RECRUITMENT BY HISTAMINE
Autore:
HE SH; PENG Q; WALLS AF;
Indirizzi:
UNIV SOUTHAMPTON,SOUTHAMPTON GEN HOSP,IMMUNOPHARMACOL GRP,CTR BLOCK SOUTHAMPTON SO16 6YD HANTS ENGLAND UNIV SOUTHAMPTON,SOUTHAMPTON GEN HOSP,IMMUNOPHARMACOL GRP SOUTHAMPTONSO16 6YD HANTS ENGLAND
Titolo Testata:
The Journal of immunology
fascicolo: 12, volume: 159, anno: 1997,
pagine: 6216 - 6225
SICI:
0022-1767(1997)159:12<6216:PIOANA>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; CHEMOTACTIC ACTIVITY; PROTEOLYTIC INACTIVATION; BRONCHOALVEOLAR LAVAGE; INFLAMMATORY RESPONSES; VASCULAR-PERMEABILITY; SYSTEMIC-ANAPHYLAXIS; CUTANEOUS RESPONSES; ASTHMATIC RESPONSES; ALLERGEN CHALLENGE;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
68
Recensione:
Indirizzi per estratti:
Citazione:
S.H. He et al., "POTENT INDUCTION OF A NEUTROPHIL AND EOSINOPHIL-RICH INFILTRATE IN-VIVO BY HUMAN MAST-CELL TRYPTASE - SELECTIVE ENHANCEMENT OF EOSINOPHIL RECRUITMENT BY HISTAMINE", The Journal of immunology, 159(12), 1997, pp. 6216-6225

Abstract

Tryptase is the most abundant protein constituent of the secretory granules of human mast cells, but little is known of the contribution ofthis serine proteinase in acute allergic reactions. We have purified tryptase from human lung tissue by immunoaffinity procedures, and haveinvestigated its potential to provoke an inflammatory infiltrate in vivo. Within 6 h of injection into the skin of guinea pigs, the accumulation of large numbers of neutrophils and eosinophils was observed, and those eosinophils closest to the injection site were partially degranulated. Similarly, injection of tryptase into the peritoneum of mice,even in quantities as low as 5 ng, stimulated the ingress of neutrophils. The response was dose dependent at 3, 6, and 16 h, with increasesin median numbers of up to 400-fold. At the later time points eosinophil numbers were increased by up to 10-fold, and there were elevationsalso in the numbers of lymphocytes and macrophages. In both models, the actions of tryptase appeared to be dependent on an intact catalyticsite. Coinjection of heparin with tryptase had relatively little effect on tryptase-induced responses. On the other hand, although histamine did not itself stimulate cell accumulation, over a range of concentrations it altered the cellular composition of the infiltrate induced by tryptase. Addition of histamine to tryptase provoked selective increases in eosinophil numbers of up to fivefold in the mouse peritoneum. Tryptase may provide an important stimulus for granulocyte recruitmentin allergic disease.

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Documento generato il 27/01/21 alle ore 02:13:14