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Titolo:
ENHANCED T-CELL ENGRAFTMENT AFTER RETROVIRAL DELIVERY OF AN ANTIVIRALGENE IN HIV-INFECTED INDIVIDUALS
Autore:
RANGA U; WOFFENDIN C; VERMA S; XU L; JUNE CH; BISHOP DK; NABEL GJ;
Indirizzi:
UNIV MICHIGAN,HOWARD HUGHES MED INST,MED CTR,1150 W MED CTR DR,4520 MSRB 1 ANN ARBOR MI 48109 UNIV MICHIGAN,HOWARD HUGHES MED INST,MED CTR ANN ARBOR MI 48109 UNIV MICHIGAN,DEPT INTERNAL MED,MED CTR ANN ARBOR MI 48109 UNIV MICHIGAN,DEPT BIOL CHEM,MED CTR ANN ARBOR MI 48109 UNIV MICHIGAN,DEPT SURG,MED CTR ANN ARBOR MI 48109 HENRY M JACKSON FDN ADVANCEMENT MIL MED,US MIL HIV RES PROGRAM BETHESDA MD 20889
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 3, volume: 95, anno: 1998,
pagine: 1201 - 1206
SICI:
0027-8424(1998)95:3<1201:ETEARD>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; TRANSDOMINANT NEGATIVE FORM; LIMITING DILUTION ANALYSIS; SINGLE-CHAIN ANTIBODY; REV ACTIVATION DOMAIN; VIRAL MESSENGER-RNA; CYSTIC-FIBROSIS; RECOMBINANT ADENOVIRUSES; CD28 COSTIMULATION; HAIRPIN RIBOZYME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
U. Ranga et al., "ENHANCED T-CELL ENGRAFTMENT AFTER RETROVIRAL DELIVERY OF AN ANTIVIRALGENE IN HIV-INFECTED INDIVIDUALS", Proceedings of the National Academy of Sciences of the United Statesof America, 95(3), 1998, pp. 1201-1206

Abstract

Intracellular expression of gene products that inhibit viral replication have the potential to complement current antiviral approaches to the treatment of AIDS, We previously have shown that a mutant inhibitory form of an essential viral protein, Rev M10, prolongs the survival of T cells transduced with a nonviral vector in HIV-infected individuals, Because these gene-modified cells were not observed in patients beyond 8 weeks, efforts were made to improve the duration of engraftment,In this study, we used retroviral vector delivery of Rev M10 to CD4(+) cells and analyzed relevant immune responses in a pilot study of three HIV seropositive patients, DNA and RNA PCR analyses revealed that cells transduced with Rev M10 retroviral vectors survived and expressedthe recombinant gene for significantly longer time periods than thosetransduced with a negative control vector in all three patients. Immune responses were not detected either to Rev M10 or to Moloney murine leukemia virus gp70 envelope protein, Rev M10-transduced cells were detected for an average of 6 months after retroviral gene transfer compared with approximate to 3 weeks for the previously reported nonviral vector delivery, These findings suggest that retroviral delivery of an antiviral gene may potentially contribute to immune reconstitution in AIDS and could provide a more effective vector to prolong survival of CD4(+) cells in HIV infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 12:12:53