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Titolo:
UNIQUE BEHAVIOR OF NUMA DURING HEAT-INDUCED APOPTOSIS OF LYMPHOCYTES
Autore:
SODJA C; WALKER PR; BROWN DL; CHALY N;
Indirizzi:
CARLETON UNIV,DEPT BIOL,1125 COLONEL BY DR OTTAWA ON K1S 5B6 CANADA CARLETON UNIV,DEPT BIOL OTTAWA ON K1S 5B6 CANADA NATL RES COUNCIL CANADA,INST BIOL SCI,APOPTOSIS RES GRP OTTAWA ON K1A0R6 CANADA UNIV OTTAWA,DEPT BIOL OTTAWA ON K1N 6N5 CANADA
Titolo Testata:
Biochemistry and cell biology
fascicolo: 4, volume: 75, anno: 1997,
pagine: 399 - 414
SICI:
0829-8211(1997)75:4<399:UBONDH>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOTIC APPARATUS PROTEIN; NUCLEAR MATRIX PROTEIN; CELL-DEATH; TOPOISOMERASE-II; DNA FRAGMENTATION; IN-VITRO; MONOCLONAL-ANTIBODIES; ELECTRON-MICROSCOPY; MOLECULAR-WEIGHT; MAMMALIAN-CELLS;
Keywords:
LYMPHOCYTES; NUMA; LAMIN B; NUCLEAR MATRIX; APOPTOSIS; HYPERTHERMIA; PROTEOLYSIS; DNA FRAGMENTATION; IMMUNOFLUORESCENCE; FLOW CYTOMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
86
Recensione:
Indirizzi per estratti:
Citazione:
C. Sodja et al., "UNIQUE BEHAVIOR OF NUMA DURING HEAT-INDUCED APOPTOSIS OF LYMPHOCYTES", Biochemistry and cell biology, 75(4), 1997, pp. 399-414

Abstract

Nuclear collapse is a key feature of apoptosis, reflecting the DNA and protein fragmentation observed biochemically. We have compared nuclear events during spontaneous and heat-induced (42 degrees C for 30 min) apoptosis at the level of individual cells, monitoring overall chromatin organization by staining with 4,6'-diamidino-2-phenylindole (DAPI), DNA cleavage by terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labelling (TUNEL), and the nuclear antigens NuMA, PI2, and lamin B by immunofluorescence microscopy. The data were correlated with analyses at the population level by flow cytometry and immunoblotting. We show that heat treatment doubles the level of apoptotic splenocytes with respect to the spontaneous apoptosis in controls within6 h. The organization and disassembly of nuclear envelope antigens isidentical during spontaneous and heat-induced apoptosis and proceeds in a temporally and spatially ordered manner relative to DNA fragmentation and chromatin collapse. In contrast, NuMA organization is affected by heat treatment, with about half of the cells containing many bright spots in addition to the usual diffuse labelling. The spots were still visible in many fully collapsed apoptotic nuclei. Further, this novel reorganization of NuMA and the hyperinduction of apoptosis by heatare correlated, suggesting that lymphocytes with reorganized NuMA aredestined to undergo apoptosis. The data also indicate that DNA fragmentation precedes extensive remodelling of the nucleoplasm in these cells, and that chromatin begins to collapse before disassembly of nuclear envelope components.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 03:39:11