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Titolo:
INCREASES IN DNA LESIONS AND THE DNA-DAMAGE INDICATOR GADD45 FOLLOWING TRANSIENT CEREBRAL-ISCHEMIA
Autore:
HOU ST; TU YH; BUCHAN AM; HUANG ZG; PRESTON E; RASQUINHA I; ROBERTSON GS; MACMANUS JP;
Indirizzi:
NATL RES COUNCIL CANADA,INST BIOL SCI,APOPTOSIS RES GRP,MONTREAL RD LABS OTTAWA ON K1A 0R6 CANADA NATL RES COUNCIL CANADA,INST BIOL SCI,APOPTOSIS RES GRP OTTAWA ON K1A0R6 CANADA UNIV CALGARY,DEPT CLIN NEUROSCI CALGARY AB T2N 1N4 CANADA UNIV OTTAWA,FAC MED,DEPT PHARMACOL OTTAWA ON K1N 6N5 CANADA
Titolo Testata:
Biochemistry and cell biology
fascicolo: 4, volume: 75, anno: 1997,
pagine: 383 - 392
SICI:
0829-8211(1997)75:4<383:IIDLAT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; MESSENGER-RNA; FOREBRAIN ISCHEMIA; GERBIL BRAIN; BETA-ACTIN; RAT-BRAIN; INTERNUCLEOSOMAL DNA; TUMOR-SUPPRESSOR; STRAND BREAKS; CELL-DEATH;
Keywords:
APOPTOSIS; DNA DAMAGE; GADD45; ISCHEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
S.T. Hou et al., "INCREASES IN DNA LESIONS AND THE DNA-DAMAGE INDICATOR GADD45 FOLLOWING TRANSIENT CEREBRAL-ISCHEMIA", Biochemistry and cell biology, 75(4), 1997, pp. 383-392

Abstract

Transient global or focal ischemia leads to the production of severaltypes of lesions in the DNA backbone including alkali-labile sites, and both single-stranded (ss) and double-stranded (ds) breaks. The ds breaks result in high molecular weight fragments of 10-50 kbp that contain both 3'- and 5'-OH end groups, suggesting that more than one endonuclease is involved. This lesioning of DNA is followed by the appearance of the damage-response indicator Gadd45 in the ischemic hemisphere following middle cerebral artery occlusion. By 6 h, gadd45 mRNA was shown to increase by semi-quantitative reverse transcriptase-polymerase chain reaction. In situ hybridization histochemistry indicated that these increases in gadd45 mRNA occurred in pyramidal neurons located on the edge of the infarcted cortex. Gadd45 immunostaining yielded similar findings with maximal protein staining detected at 18 h after occlusion. In neurons, in the infarct core with frank DNA fragmentation shown by in situ TdT-mediated dUTP-biotin nick end labeling (TUNEL) at 24 h, the Gadd45 immunostaining was not visible. Taken together, these findings suggest that Gadd45 responds to DNA damage following ischemia as part of a repair response mounted by brain cells attempting to survive the insult.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 15:04:51