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Titolo:
ASSESSMENT OF MESSENGER-RNA OF BETA-1-]4-N-ACETYLGALACTOSAMINYLTRANSFERASE AS A MOLECULAR MARKER FOR METASTATIC MELANOMAL
Autore:
KUO CT; BOSTICK PJ; IRIE RF; MORTON DL; CONRAD AJ; HOON DSB;
Indirizzi:
JOHN WAYNE CANC INST,DEPT MOL ONCOL,2200 SANTA MONICA BLVD SANTA MONICA CA 90404 JOHN WAYNE CANC INST,DEPT MOL ONCOL SANTA MONICA CA 90404 JOHN WAYNE CANC INST,DEPT BIOTECHNOL SCI SANTA MONICA CA 90404 NATL INST GENET SANTA MONICA CA 90404
Titolo Testata:
Clinical cancer research
fascicolo: 2, volume: 4, anno: 1998,
pagine: 411 - 418
SICI:
1078-0432(1998)4:2<411:AOMOB>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN MONOCLONAL-ANTIBODY; ACTIVE SPECIFIC IMMUNOTHERAPY; GANGLIOSIDE GM2; MALIGNANT-MELANOMA; CELL-LINES; EXPRESSION; GD2; CANCER; ANTIGEN; SURVIVAL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
C.T. Kuo et al., "ASSESSMENT OF MESSENGER-RNA OF BETA-1-]4-N-ACETYLGALACTOSAMINYLTRANSFERASE AS A MOLECULAR MARKER FOR METASTATIC MELANOMAL", Clinical cancer research, 4(2), 1998, pp. 411-418

Abstract

Gangliosides GM2 [GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-4Glc beta 1-1Cer] and GD2 [GalNAc beta 1-4(NeuAc alpha 2-8NeuAc alpha 2-3)Gal beta 1-4Glc beta 1-1Cer] are cell surface tumor-associated antigens and have been demonstrated to be important markers of human malignant melanoma progression, Expression of these glycolipid antigens on melanoma tissues can be assessed by immunohistochemistry or biochemical analysis, These methodologies, however, are not logistically practical or sensitive for testing metastatic melanoma cells in blood or in tissue biopsies, In the present study, we hypothesized that the enzyme involved in GM2 and GD2 synthesis, beta 1-->4-N-acetylgalactosaminyltransferase (beta 1-->4GalNac-T), can be a useful marker for detection of occult metastatic melanoma, A reverse transcription PCR and Southern blot assay to detect beta 1-->4GalNac-T mRNA expression was developed, beta 1-->4GalNac-T mRNA was detected in all 13 melanoma cell lines tested, Metastatic melanoma of lymph nodes and different organ sites expressedbeta 1-->4GalNac-T mRNA at various levels, Detection sensitivity of the reverse transcription PCR assay was 1 ng of total RNA extracted from tumor specimens and approximately 5 melanoma cells in 20 million normal donor peripheral blood lymphocytes, In assessment of blood from 126 melanoma patients, beta 1-->4GalNac-T mRNA was more frequently foundin advanced-stage melanomas and in patients showing more aggressive tumor progression, Normal donor blood samples (n = 37) were all negative for beta 1-->GalNac-T mRNA expression, These results suggest that beta 1-->4GalNac-T mRNA is a promising molecular marker for detecting melanoma cells, characterizing antigen expression, and monitoring tumor progression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:10:52