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Titolo:
A GENETICALLY-ENGINEERED INCREASE IN FATTY-ACID UNSATURATION IN SYNECHOCOCCUS SP. PCC-7942 ALLOWS EXCHANGE OF D1 PROTEIN FORMS AND SUSTENANCE OF PHOTOSYSTEM-II ACTIVITY AT LOW-TEMPERATURE
Autore:
SIPPOLA K; KANERVO E; MURATA N; ARO EM;
Indirizzi:
UNIV TURKU,DEPT BIOL FIN-20014 TURKU FINLAND UNIV TURKU,DEPT BIOL FIN-20014 TURKU FINLAND NATL INST BASIC BIOL,DEPT REGULAT BIOL OKAZAKI AICHI 444 JAPAN
Titolo Testata:
European journal of biochemistry
fascicolo: 3, volume: 251, anno: 1998,
pagine: 641 - 648
SICI:
0014-2956(1998)251:3<641:AGIIFU>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SP STRAIN PCC-7942; HIGH LIGHT-INTENSITY; BLUE-GREEN-ALGAE; CYANOBACTERIUM SYNECHOCOCCUS; CHILLING SENSITIVITY; MEMBRANE-LIPIDS; OVER-PRODUCTION; GENE FAMILY; PSBA GENES; 2 FORMS;
Keywords:
MEMBRANE LIPID; PSBA GENE EXPRESSION; SYNECHOCOCCUS; PHOTOSYSTEM II; D1 REACTION-CENTER PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
K. Sippola et al., "A GENETICALLY-ENGINEERED INCREASE IN FATTY-ACID UNSATURATION IN SYNECHOCOCCUS SP. PCC-7942 ALLOWS EXCHANGE OF D1 PROTEIN FORMS AND SUSTENANCE OF PHOTOSYSTEM-II ACTIVITY AT LOW-TEMPERATURE", European journal of biochemistry, 251(3), 1998, pp. 641-648

Abstract

Photosystem II (PSII)-reaction-center protein D1 is encoded by three psbA genes in Synechococcus sp. PCC 7942. The psbA gene encodes D1 form I (D1:1) and the psbAIII and pshAlll genes encode the transiently expressed D1 form II (D1:2). We have studied the role of membrane-lipid unsaturation in the expression of psbA genes at low temperature, usinga Synechococcus transformant with an increased unsaturation level of membrane lipids.Transfer of the cells from 32 degrees C to 25 degrees C under growth light resulted in the exchange of D1:1, the prevailing form, for D1:2 in the wild- type bacterium and the transformant, with no loss of PSII activity.Lowering the temperature further to 18 degrees C caused a drastic decrease in PSII activity in the wild-type bacterium, whereas the transformant was much less affected. Similar decreases in psbAI transcripts and loss of D1 : 1 occurred in both strains at 18 degrees C, with concomitant accumulation of psbAII/III transcripts,the latter event being especially prominent in the wild-type bacterium. However, the wild-type bacterium was incapable of accumulating D1:2to compensate for the loss of D1:l, which resulted in disassembly of PSII at low temperature. These results imply translational rather thantranscriptional regulation of psbA gene expression in Synechococcus 7942 at low temperature, and demonstrate the crucial role of the degreeof membrane-lipid unsaturation in promotion of exchange of the D1 protein forms and thus the sustenance of PSII function at low temperature.

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Documento generato il 20/09/20 alle ore 07:58:46