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Titolo:
THE DERIVATION AND CHARACTERIZATION OF STROMAL CELL-LINES FROM THE BONE-MARROW OF P53(- -) MICE - NEW INSIGHTS INTO OSTEOBLAST AND ADIPOCYTE DIFFERENTIATION/
Autore:
THOMPSON DL; LUM KD; NYGAARD SC; KUESTNER RE; KELLY KA; GIMBLE JM; MOORE EE;
Indirizzi:
ZYMOGENET INC,1201 EASTLAKE AVE E SEATTLE WA 98102 ZYMOGENET INC SEATTLE WA 98102 OKLAHOMA MED RES FDN OKLAHOMA CITY OK 73104
Titolo Testata:
Journal of bone and mineral research
fascicolo: 2, volume: 13, anno: 1998,
pagine: 195 - 204
SICI:
0884-0431(1998)13:2<195:TDACOS>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
P53-DEFICIENT MICE; STEM-CELLS; P53; CULTURE; GROWTH; IMMORTALIZATION; CONVERSION; ASTROCYTES; PROTEIN; MOUSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
D.L. Thompson et al., "THE DERIVATION AND CHARACTERIZATION OF STROMAL CELL-LINES FROM THE BONE-MARROW OF P53(- -) MICE - NEW INSIGHTS INTO OSTEOBLAST AND ADIPOCYTE DIFFERENTIATION/", Journal of bone and mineral research, 13(2), 1998, pp. 195-204

Abstract

We have derived a series of clonal cell lines from the bone marrow ofp53(-/-)mice that represent different stages of osteoblast and adipocyte differentiation, All cell lines show indefinite growth potential (>300 population doublings) and have generation times of 12-20 h, Thesecell lines have been grouped into three categories, The least mature clones are heterogeneous and appear to contain a subpopulation of stemcells, which can spontaneously generate foci that contain either adipocytes or mineralizing osteoblasts, The second category of clones are homogenous and clearly correspond to mature osteoblasts because they express high levels of the anticipated osteoblastic markers in a stablefashion and cannot differentiate into adipocytes even in the presenceof inducers, The clones in the third category are the most unique, Initially they appeared to correspond to mature osteoblasts because theyexpress alkaline phosphatase in a homogenous manner, secrete type I collagen, show a significant cyclic adenosine monophosphate response toparathyroid hormone, secrete osteocalcin, and mineralize extensively after only 4-7 days, However, in contrast to the mature osteoblasts, these clones can be induced to undergo massive adipocyte differentiation, and this differentiation is accompanied by the complete loss of expression of all osteoblastic markers except alkaline phosphatase. Theseobservations indicate that some cells that have acquired all of the characteristics of mature osteoblasts can be diverted to the adipocyte pathway. Further characterization of these clones may be particularly relevant to osteoporotic conditions where increased adipocyte formation appears to occur at the expense of osteoblast formation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:47:23