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Titolo:
PRESENCE OF GROWTH-HORMONE SECRETAGOGUE RECEPTOR MESSENGER-RIBONUCLEIC-ACID IN HUMAN PITUITARY-TUMORS AND RAT GH(3) CELLS
Autore:
ADAMS EF; HUANG B; BUCHFELDER M; HOWARD A; SMITH RG; FEIGHNER SD; VANDERPLOEG LHT; BOWERS CY; FAHLBUSCH R;
Indirizzi:
UNIV ERLANGEN NURNBERG,NEUROCHIRURG KLIN,NEUROENDOKRINOL LAB,DEPT NEUROSURG,SCHWABACHANLAGE 6 D-91054 ERLANGEN GERMANY MERCK RES LABS RAHWAY NJ 07065 TULANE UNIV,MED CTR,DEPT MED NEW ORLEANS LA 70112
Titolo Testata:
The Journal of clinical endocrinology and metabolism
fascicolo: 2, volume: 83, anno: 1998,
pagine: 638 - 642
SICI:
0021-972X(1998)83:2<638:POGSRM>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYROTROPIN-RELEASING-HORMONE; BIOCHEMICAL CHARACTERISTICS; ACROMEGALIC PATIENTS; PROLACTIN SECRETION; INVIVO ACTIVITY; IN-VITRO; PEPTIDE; HEXAPEPTIDE; ASSOCIATION; MUTATIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
E.F. Adams et al., "PRESENCE OF GROWTH-HORMONE SECRETAGOGUE RECEPTOR MESSENGER-RIBONUCLEIC-ACID IN HUMAN PITUITARY-TUMORS AND RAT GH(3) CELLS", The Journal of clinical endocrinology and metabolism, 83(2), 1998, pp. 638-642

Abstract

A novel G(11)-protein-coupled receptor specific for synthetic GH-releasing peptides (GHRPs) has recently been cloned and sequenced. Two forms exist, types 1a and 1b, the latter of which is biologically inactive. Using RT-PCR, we looked for the presence in tumorous pituitary cells of messenger ribonucleic acid (mRNA) for this novel GH secretagogue receptor (GHS-R). Both subtypes of GHS-R mRNA were detected in all sixhuman pituitary somatotropinomas removed from patients with acromegaly. In culture, four of the tumors exhibited strong responses to GHRP-2in terms of both phosphatidylinositol (PI) hydrolysis and GH secretion, but two were resistant. There was no apparent difference in the type 1a and type 1b expression pattern, as judged by RT-PCR, between responsive and nonresponsive tumors. Similarly, the rat pituitary tumor cell line, GH(3), was found to express GHS-R mRNA, although these cells also did not respond to GHRPs. RT-PCR failed to detect GHS-R mRNA in eight functionless human pituitary tumors. In contrast, prolactinomas were found to express the receptor and, in culture, significant stimulation of PRL secretion and PI hydrolysis occurred in two of three tumors tested. These results demonstrate that tumorous somatotrophs expressthe GHS-R gene and that the occasionally observed nonresponsiveness of somatotropinomas to GHRPs is not due to the absence of the biologically active type la receptor. Additionally, human pituitary prolactinomas also express GHS-R and are able to respond to GHRPs in terms of PI hydrolysis and PRL secretion. In contrast, GWS-R gene expression does not appear to be associated with human functionless pituitary tumors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 22:45:58