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Titolo:
A NOVEL CENTROMERE MONOSPECIFIC SERUM TO A HUMAN AUTOEPITOPE ON THE HISTONE H3-LIKE PROTEIN CENP-A
Autore:
VALDIVIA MM; FIGUEROA J; IGLESIAS C; ORTIZ M;
Indirizzi:
UNIV CADIZ,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOL CADIZ 11510 SPAIN
Titolo Testata:
FEBS letters
fascicolo: 1, volume: 422, anno: 1998,
pagine: 5 - 9
SICI:
0014-5793(1998)422:1<5:ANCMST>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
APOPTOTIC THYMOCYTES; MOLECULAR-CLONING; KINETOCHORE; CLEAVAGE; CELLS; SCLERODERMA; AUTOANTIGEN; DEGRADATION; ANTIGEN; DOMAIN;
Keywords:
CENTROMERE; CENP-A; AUTOANTIGEN; ANTIPEPTIDE SERUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
M.M. Valdivia et al., "A NOVEL CENTROMERE MONOSPECIFIC SERUM TO A HUMAN AUTOEPITOPE ON THE HISTONE H3-LIKE PROTEIN CENP-A", FEBS letters, 422(1), 1998, pp. 5-9

Abstract

Centromere autoantibodies are commonly found in the serum of patientswith some systemic autoimmune diseases, Previous studies have shown that a major human centromere autoantigen is the histone H3-like protein CENP-A. Although the human cDNA has been cloned, native CENP-A has been neither isolated nor expressed in Escherichia coli, and specific antibodies to this chromatin-associated centromere protein are not available yet. In this report, a highly charged peptide on CENP-A (residues 3-17) was used to generate a monospecific antibody that reacts by immunoblots with the 17 kDa centromeric protein, Immunofluorescence analysis showed reactivity of this anti-CENP-A serum in several but not all mammalian culture cells analyzed, suggesting that the sequence of this histone-like centromere protein could be more variable throughout evolution than originally thought, Selective extractions of human placenta nuclear proteins and immunoblot analysis indicated that CENP-A behaves in a similar way to the core histone polypeptides after nuclease digestion of chromatin, Also, immunoblot analysis demonstrated that the CENP-A peptide used as immunogen is a target region on the CENP-A molecule in several but not all CREST patients analyzed with high titersof autoantibodies to the centromere. Lastly, we found that in Jurkat cells induced to apoptosis, CENP-A remains associated with the centromere, in contrast to other human autoantigens studied during apoptosis. (C) 1998 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/05/20 alle ore 19:39:56