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Titolo:
ROUND-SPOTTED PUFFERFISH (TETRAODON FLUVIATILIS) SNF5 GENE IS ORIENTED IN A TAIL-TO-TAIL MANNER WITH THE SET GENE WHICH ENCODES AN INHIBITOR OF PROTEIN PHOSPHATASE 2A
Autore:
YAO CW; LEU JH; CHIN C; CHOU CK; HUANG CJ;
Indirizzi:
ACAD SINICA,INST BIOL CHEM TAIPEI TAIWAN ACAD SINICA,INST BIOL CHEM TAIPEI TAIWAN NATL DEF MED CTR,GRAD INST LIFE SCI TAIPEI TAIWAN NATL DEF MED CTR,INST PREVENT MED TAIPEI TAIWAN VET GEN HOSP,DEPT MED RES TAIPEI 11217 TAIWAN
Titolo Testata:
DNA and cell biology
fascicolo: 1, volume: 17, anno: 1998,
pagine: 69 - 82
SICI:
1044-5498(1998)17:1<69:RP(FSG>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; TRANSCRIPTIONAL ACTIVATORS; MYELOID LEUKEMOGENESIS; NUCLEAR-LOCALIZATION; PUTATIVE ONCOGENE; GENOMIC STRUCTURE; MESSENGER-RNA; EXPRESSION; BINDING; YEAST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
C.W. Yao et al., "ROUND-SPOTTED PUFFERFISH (TETRAODON FLUVIATILIS) SNF5 GENE IS ORIENTED IN A TAIL-TO-TAIL MANNER WITH THE SET GENE WHICH ENCODES AN INHIBITOR OF PROTEIN PHOSPHATASE 2A", DNA and cell biology, 17(1), 1998, pp. 69-82

Abstract

The round-spotted pufferfish Tetraodon fluviatilis has a genome size of 380 Mb which is slightly smaller than that of another pufferfish Fugu rubripes rubripes (Fugu). Due to its compact genome and small introns, Fugu has been introduced as a model for genome studies. Recently, the round-spotted pufferfish has also been proposed as a nem model forgenome studies because of the ease in obtaining material and high-sequence homology to that of Fugu. In this study, me have cloned and characterized the snf5 and set genes from the round-spotted pufferfish. The snf5 gene is composed of 9 exons spanning about 2.9 kb whereas the set gene consists of 8 exons spanning about 2.7 kb. They are linked in a tail-to-tail manner with an intergenic region of about 6.5 kb. So far, the genomic structures of human snf5 and set genes are unknown. Based on our data, the pufferfish SNF5 and SET display high amino acid sequence identity (>90%) with the respective human genes. By primer extension and sequence analysis, we found that putative promoter region ofthe snf5 gene contains a typical TATA box and numerous potential binding sites for transcription factors including AP1, AP2, AP3, c-Myb, HNF-5, and NF-IL6. As for the set gene, its promoter region does not have any TATA or CCAAT motif and contains a few potential binding sites for transcriptional factors such as c-Myb and gamma-IRE. When these promoter regions were placed upstream of the CAT reporter gene and transfected into a carp CF cell line, the 5'-upstream 1.6-kb DNA fragment ofthe snf5 gene displayed stronger promoter activity, approximately three-fold higher than that of the 5'-upstream 1.3 kb DNA fragment of theset gene. By transient expression and immunofluorescent staining, we also showed that the pufferfish SNF5 and SET are nuclear proteins, consistent with their postulated roles as transcriptional factors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 04:14:49