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Titolo:
GENE-EXPRESSION OF THE RENIN-ANGIOTENSIN SYSTEM IN HUMAN KIDNEY
Autore:
LAI KN; LEUNG JCK; LAI KB; TO WY; YEUNG VTF; LAI FMM;
Indirizzi:
UNIV HONG KONG,QUEEN MARY HOSP,DEPT MED,PROFESSORIAL BLOCK POKFULAM PEOPLES R CHINA CHINESE UNIV HONG KONG,PRINCE WALES HOSP,DEPT MED HONG KONG HONG KONG CHINESE UNIV HONG KONG,PRINCE WALES HOSP,CLIN IMMUNOL UNIT HONG KONG HONG KONG CHINESE UNIV HONG KONG,PRINCE WALES HOSP,DEPT PATHOL HONG KONG HONG KONG
Titolo Testata:
Journal of hypertension
fascicolo: 1, volume: 16, anno: 1998,
pagine: 91 - 102
SICI:
0263-6352(1998)16:1<91:GOTRSI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; PROXIMAL TUBULE; CELLS; RAT; DISEASE; GROWTH;
Keywords:
RENIN; ANGIOTENSINOGEN; ANGIOTENSIN CONVERTING ENZYME; MESSENGER-RNA; HUMAN GLOMERULUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
K.N. Lai et al., "GENE-EXPRESSION OF THE RENIN-ANGIOTENSIN SYSTEM IN HUMAN KIDNEY", Journal of hypertension, 16(1), 1998, pp. 91-102

Abstract

Background Immunocytochemical studies have revealed that all components of the renin-angiotensin system are widely distributed in human tissues yet the information on the gene expression of the renin-angiotensin system in various types of cell remains scarce. Objective We explored the presence of a local renin-angiotensin system in human kidney. Methods We sought to determine the presence of messenger RNA (mRNA) encoding for renin, angiotensinogen, and angiotensin converting enzyme (ACE) in cultured human glomerular cells and human umbilical vein endothelial cells using a two-step polymerase chain reaction. The gene expression of the renin-angiotensin system in normal human kidney and in diseased kidney was studied by in-situ hybridization using synthetic oligonucleotides. Results By using a two-step polymerase chain reaction, renin, angiotensinogen, and ACE mRNA were found in cultured mesangial and epithelial cells but only ACE mRNA was present in human umbilical vein endothelial cells. Renin mRNA was detected in juxtaglomerular granular cells and also in glomerular and tubular epithelia in normal kidney by in-situ hybridization. A similar tubular, but not mesangial, distribution was found with angiotensinogen and ACE mRNA. In contrast, stronger signals for renin, angiotensinogen and ACE mRNA were detected in mesangial and epithelial cells of kidney tissues from hypertensive patients and from patients with renal pathology characterized by mesangial proliferation (immunoglobulin A nephropathy, diabetes mellitus, or lupus nephritis). Conclusions That gene expression of the renin-angiotensin system occurs in resident glomerular cells supports the hypothesis that there is a local renin-angiotensin system in human kidney. Our findings support the previous speculation that the renin-angiotensin system could be a local factor involved in the progression of chronic renal failure and consequent development of hypertension. (C) 1998 Rapid Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 22:19:51