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Titolo:
DISTINCT REQUIREMENTS FOR CHROMATIN ASSEMBLY IN TRANSCRIPTIONAL REPRESSION BY THYROID-HORMONE RECEPTOR AND HISTONE DEACETYLASE
Autore:
WONG JM; PATTERTON D; IMHOF A; GUSCHIN D; SHI YB; WOLFFE AP;
Indirizzi:
NICHHD,MOL BIOL SECT,MOL EMBRYOL LAB,NIH,BLDG 18T,ROOM 106 BETHESDA MD 20892 NICHHD,MOL BIOL SECT,MOL EMBRYOL LAB,NIH BETHESDA MD 20892 NICHHD,UNIT MOL MORPHOGENESIS,NIH BETHESDA MD 20892
Titolo Testata:
EMBO journal
fascicolo: 2, volume: 17, anno: 1998,
pagine: 520 - 534
SICI:
0261-4189(1998)17:2<520:DRFCAI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATION FUNCTION AF-2; YEAST ALPHA-2 REPRESSOR; RETINOIC ACID RECEPTOR; TUMOR VIRUS PROMOTER; XENOPUS-LAEVIS; SACCHAROMYCES-CEREVISIAE; NUCLEAR RECEPTORS; NUCLEOSOMAL DNA; PHO5 PROMOTER; IN-VIVO;
Keywords:
CHROMATIN DISRUPTION; HISTONE ACETYLATION; NUCLEAR RECEPTOR; TRANSCRIPTION FACTOR ACETYLATION; TRANSCRIPTIONAL CONTROL;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
116
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Wong et al., "DISTINCT REQUIREMENTS FOR CHROMATIN ASSEMBLY IN TRANSCRIPTIONAL REPRESSION BY THYROID-HORMONE RECEPTOR AND HISTONE DEACETYLASE", EMBO journal, 17(2), 1998, pp. 520-534

Abstract

Histone deacetylase and chromatin assembly contribute to the control of transcription of the Xenopus TR beta A gene promoter by the heterodimer of Xenopus thyroid hormone receptor and 9-cis retinoic acid receptor (TR-RXR), Addition of the histone deacetylase inhibitor Trichostatin A (TSA) relieves repression of transcription due to chromatin assembly following microinjection of templates into Xenopus oocyte nuclei, and eliminates regulation of transcription by TR-RXR. Expression of Xenopus RPD3p, the catalytic subunit of histone deacetylase, represses the TR beta A promoter, but only after efficient assembly of the template into nucleosomes, In contrast, the unliganded TR-RXR represses templates only partially assembled into nucleosomes; addition of TSA also relieves this transcriptional repression, This result indicates the distinct requirements for chromatin assembly in mediating transcriptional repression by the deacetylase alone, compared with those needed in the presence of unliganded TR-RXR. In addition, whereas hormone-bound TR-RXR targets chromatin disruption as assayed through changes in minichromosome topology and loss of a regular nucleosomal ladder on micrococcal nuclease digestion, addition of TSA relieves transcriptional repression but does not disrupt chromatin, Thus, TR-RXR can facilitate transcriptional repression in the absence of hormone through mechanisms in addition to recruitment of deacetylase, and disrupts chromatin structure through mechanisms in addition to the inhibition or release of deacetylase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:15:37