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Titolo:
DETECTION OF BOVINE IMMUNODEFICIENCY-LIKE VIRUS-INFECTION IN EXPERIMENTALLY INFECTED CALVES
Autore:
BARON T; BETEMPS D; MALLET F; CHEYNET V; LEVY D; BELLI P;
Indirizzi:
LAB PATHOL BOVINE,CNEVA,31 AVE TONY GARNIER,BP 7033 F-69342 LYON 07 FRANCE CTR NATL ETUDES VET & ALIMENTAIRES LYON FRANCE ECOLE NORMALE SUPER,CNRS BIOMERIEUX UMR 103 LYON FRANCE URA INRA IMMUNOPATHOL CELLULAIRE & MOL MAISONS ALFORT FRANCE
Titolo Testata:
Archives of virology
fascicolo: 1, volume: 143, anno: 1998,
pagine: 181 - 189
SICI:
0304-8608(1998)143:1<181:DOBIVI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; MULTIPLE RETROVIRAL INFECTIONS; BIOLOGICALLY-ACTIVE PROVIRUSES; MOLECULAR-CLONING; LYMPHOID-TISSUE; LEUKEMIA-VIRUS; CATTLE; TYPE-1; ASSAY; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
T. Baron et al., "DETECTION OF BOVINE IMMUNODEFICIENCY-LIKE VIRUS-INFECTION IN EXPERIMENTALLY INFECTED CALVES", Archives of virology, 143(1), 1998, pp. 181-189

Abstract

Detection of BIV virus infection by serological means, PCR and virus isolation in experimentally infected calves is described. Viral sequences were specifically detected by PCR in peripheral blood mononuclear cells (PBMCs), with primer systems located in the gag, pot and tat regions of the viral genome. An enzyme-linked oligosorbent assay (ELOSA) in microtiter plates is described; for the detection of PCR products, the sensitivity of which was shown to be comparable to that of membrane hybridization detection. Serological response of the animals againstthe BTV p26 protein was shown, using a recombinant fusion protein ((His)(6)p26) expressed in E. coli and purified by metal affinity chromatography, in ELISA and Western blot studies. The presence of infectiousvirus was demonstrated by its rescue, by virus isolation in cell cultures, from PBMCs during a one year follow-up.

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Documento generato il 28/11/20 alle ore 21:49:14