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Titolo:
RAPD PCR-BASED DIFFERENTIATION OF XANTHOMONAS-CAMPESTRIS PV. PHASEOLIAND XANTHOMONAS-CAMPESTRIS PV. PHASEOLI VAR. FUSCANS
Autore:
BIRCH PRJ; HYMAN LJ; TAYLOR R; OPIO AF; BRAGARD C; TOTH IK;
Indirizzi:
SCOTTISH CROP RES INST,DEPT FUNGAL & BACTERIAL PLANT PATHOL DUNDEE DD2 5DA SCOTLAND SCOTTISH CROP RES INST,DEPT FUNGAL & BACTERIAL PLANT PATHOL DUNDEE DD2 5DA SCOTLAND HORT & FOOD RES INST NEW ZEALAND LTD,MT ALBERT RES CTR AUCKLAND NEW ZEALAND KAWANDA RES STN KAMPALA UGANDA
Titolo Testata:
European journal of plant pathology
fascicolo: 9, volume: 103, anno: 1997,
pagine: 809 - 814
SICI:
0929-1873(1997)103:9<809:RPDOXP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PATHOGENIC VARIATION; BACTERIAL-BLIGHT; COMMON; PATHOVARS; STRAINS; BEANS; ACID;
Keywords:
RAPD PCR; XANTHOMONAS CAMPESTRIS PV. PHASEOLI; COMMON BACTERIAL BLIGHT; FUSCOUS BLIGHT;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
P.R.J. Birch et al., "RAPD PCR-BASED DIFFERENTIATION OF XANTHOMONAS-CAMPESTRIS PV. PHASEOLIAND XANTHOMONAS-CAMPESTRIS PV. PHASEOLI VAR. FUSCANS", European journal of plant pathology, 103(9), 1997, pp. 809-814

Abstract

A RAPD PCR-based method was used to differentiate between isolates ofXanthomonas campestris pv. phaseoli and Xanthomonas campestris pv. phaseoli var. fuscans. Using random primer OP-G11, a single, high intensity band of 820 bp was amplified from DNAs of all X. c. pv. phaseoli var. fuscans isolates, while multiple amplification products of varyingsizes were generated from X. c. pv. phaseoli DNAs. Whereas RAPD PCR differentiation gave an unambiguous result in under 4 h, standard differentiation by recording the production of a brown pigment by X. c. pv.phaseoli var. fuscans isolates took up to 7 days and showed variationboth between isolates and between media. The unequivocal nature of the RAPD PCR method was demonstrated when isolate 408, originally classified as X. c. pv, phaseoli var. fuscans, failed to produce the 820 bp band typical of X. c. pv. phaseoli var. fuscans isolates, and after also failing to produce a brown pigment, was re-classified as X. c. pv. phaseoli.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 02:44:53