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Titolo:
APOPTOSIS AND BCL-2 PROTEIN-CHANGES IN L1210 LEUKEMIC-CELLS EXPOSED TO OXIDATIVE STRESS
Autore:
ZIMOWSKA W; MOTYL T; SKIERSKI J; BALASINSKA B; PLOSZAJ T; ORZECHOWSKI A; FILIPECKI M;
Indirizzi:
AGR UNIV WARSAW,FAC VET MED,DEPT ANIM PHYSIOL,NOWOURSYNOWSKA 166 PL-02787 WARSAW POLAND AGR UNIV WARSAW,FAC VET MED,DEPT ANIM PHYSIOL PL-02787 WARSAW POLAND DRUG INST,FLOW CYTOMETRY LAB WARSAW POLAND AGR UNIV WARSAW,DEPT PLANT GENET & BREEDING HORT WARSAW POLAND
Titolo Testata:
Apoptosis
fascicolo: 6, volume: 2, anno: 1997,
pagine: 529 - 539
SICI:
1360-8185(1997)2:6<529:AABPIL>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIOXIDANT DEFENSE; DEATH; INHIBITION; MECHANISM; RADICALS; ACID;
Keywords:
AAPH; APOPTOSIS; BCL-2; FREE RADICALS; LEUKEMIC CELLS; OXIDATIVE STRESS;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
W. Zimowska et al., "APOPTOSIS AND BCL-2 PROTEIN-CHANGES IN L1210 LEUKEMIC-CELLS EXPOSED TO OXIDATIVE STRESS", Apoptosis, 2(6), 1997, pp. 529-539

Abstract

The aim of this study was to explore the dose-and time-dependent effects of hydrophilic peroxyl radical initiator 2,2'azobis(2amidinopropane)dihydrochloride (AAPH) on apoptosis, and on expression of Bcl-2 in L1210 leukaemic cells. We observed a progressive increase of intracellular concentration of oxygen free radicals (OFR), manifested by the rise of 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) oxidation, during 24 h of cells exposure to AAPH, Oxidative stress was associated with peroxidation of cellular lipids, which was demonstrated by the measurement of thiobarbituric acid-reactive substances and conjugated dienes, Analysis of cell viability by the use of trypan blue exclusion method revealed that AAPH reduced the ability of L1210 cells to multiply or survive, AAPH increased the number of leukaemic cells with typical features of apoptosis like condensation of chromatin, pyknosis and fragmentation of nucleus, followed by secondary necrosis. A characteristic internucleosomal DNA cleavage, visualizedas a DNA 'ladder' consisting of fragments that are multi ples of 180-200 bp was also observed. The intensity of apoptosis was dependent on AAPH concentration: time of cell exposure and the availability of growth factors and nutrients in extracellular environment (FCS concentration), The novel observation is the increase of Bcl-2 level in L1210 leukaemic cells surviving an oxidative stress. The level of Bcl-2 proteinsignificantly rose with increasing AAPH concentration, and time of cell exposure to this oxidant. This phenomenon could be the result of: (1) negative selection of cells with the lowest expression of bcl-2, being more susceptible to oxidative stress and (2) increased synthesis and/or decreased;ed degradation of Bcl-2 protein as an adaptation to continuous OFR loading, In contrast to growth-promoting medium (10% FCS/RPMI), the maintenance medium (2% FCS/RPMI) did not cover cell requirements for progressive Bcl-2 increase at the highest AAPH concentration(2 mM) applied in this study. Several observations indicate that the increased Bcl-2 level in surviving L1210 leukaemic cells exposed to oxidative stress is a symptom of their natural defence against cellular lipids peroxidation and apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:11:46