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Titolo:
PROLIFERATION AND HYPOXIA IN HUMAN SQUAMOUS-CELL CARCINOMA OF THE CERVIX - FIRST REPORT OF COMBINED IMMUNOHISTOCHEMICAL ASSAYS
Autore:
KENNEDY AS; RALEIGH JA; PEREZ GM; CALKINS DP; THRALL DE; NOVOTNY DB; VARIA MA;
Indirizzi:
UNIV N CAROLINA,SCH MED,DEPT RADIAT ONCOL,CAMPUS BOX 7512 CHAPEL HILLNC 27599 UNIV N CAROLINA,SCH MED,DEPT GYNECOL ONCOL CHAPEL HILL NC 00000 UNIV N CAROLINA,SCH MED,DEPT PATHOL CHAPEL HILL NC 00000 N CAROLINA STATE UNIV,COLL VET MED RALEIGH NC 00000
Titolo Testata:
International journal of radiation oncology, biology, physics
fascicolo: 4, volume: 37, anno: 1997,
pagine: 897 - 905
SICI:
0360-3016(1997)37:4<897:PAHIHS>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPONTANEOUS CANINE TUMORS; METABOLISM-INDUCED BINDING; INTRINSIC RADIOSENSITIVITY; MULTICELLULAR SPHEROIDS; RADIATION-THERAPY; MISONIDAZOLE; PIMONIDAZOLE; TISSUES; MARKER; KI-67;
Keywords:
HYPOXIA; PROLIFERATION; HYPOXIA MARKER; PIMONIDAZOLE; MIB-1; PCNA; CERVIX CARCINOMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
A.S. Kennedy et al., "PROLIFERATION AND HYPOXIA IN HUMAN SQUAMOUS-CELL CARCINOMA OF THE CERVIX - FIRST REPORT OF COMBINED IMMUNOHISTOCHEMICAL ASSAYS", International journal of radiation oncology, biology, physics, 37(4), 1997, pp. 897-905

Abstract

Purpose: To characterize the distribution of hypoxia and proliferation in human squamous cell carcinoma of the cervix via an immunohistochemical approach prior to initiation of therapy, Methods and Materials: Patients with primary squamous cell carcinoma of the cervix uteri received a single infusion of the 2-nitroimidazole, pimonidazole (0.5 g/m(2) i.v.), and 24 h later punch biopsies of the primary tumor were taken, Tissue was formalin fixed, paraffin embedded, and sectioned for immunohistochemistry. Hypoxia was detected by monoclonal antibody bindingto adducts of reductively activated pimonidazole in malignant cells, Staining for endogenous MIB-1 and PCNA was detected in tumor cells viacommercially available monoclonal antibodies, Point counting was usedto quantitate the fraction of tumor cells immunostained for MIB-1, PCNA, and hypoxia marker binding, Results: Immunostaining for pimonidazole binding was distant from blood vessels, There was no staining in necrotic regions, and only minimal nonspecific staining, mostly in keratin, In general, cells immunostaining for MIB-1 and PCNA did not immunostain for pimonidazole binding, Cells immunostaining for MIB-1 and PCNA showed no obvious geographic predilection such as proximity to vasculature, Quantitative comparison showed an inverse relationship betweenhypoxia marker binding and proliferation, Conclusions: Immunohistochemical staining for pimonidazole binding is consistent with the presence of hypoxic cells in human tumors and may be useful for estimating tumor hypoxia prior to radiation therapy, Immunostaining for pimonidazole binding is an ideal complement to immunohistochemical assays for endogenous proliferation markers allowing for comparisons of tumor hypoxia with other physiological parameters, These parameters might be used to select patients for radiation protocols specifically designed to offset the negative impact of hypoxia and/or proliferation on therapy, The inverse relationship between pimonidazole binding and proliferationmarkers is a preliminary result requiring verification, (C) 1997 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 16:20:19