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Titolo:
MEASUREMENT OF NATURAL-RUBBER LATEX ALLERGEN LEVELS IN MEDICAL GLOVESBY ALLERGEN-SPECIFIC IGE-ELISA INHIBITION, RAST INHIBITION, AND SKIN PRICK TEST
Autore:
PALOSUO T; MAKINENKILJUNEN S; ALENIUS H; REUNALA T; YIP E; TURJANMAA K;
Indirizzi:
NATL PUBL HLTH INST,IMMUNOBIOL LAB,MANNERHEIMINTIE 166 FIN-00300 HELSINKI FINLAND UNIV HOSP SKIN & ALLERG DIS HELSINKI FINLAND RUBBER RES INST KUALA LUMPUR MALAYSIA UNIV TAMPERE,SCH MED FIN-33101 TAMPERE FINLAND TAMPERE UNIV HOSP,DEPT DERMATOL TAMPERE FINLAND
Titolo Testata:
Allergy
fascicolo: 1, volume: 53, anno: 1998,
pagine: 59 - 67
SICI:
0105-4538(1998)53:1<59:MONLAL>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEVEA-BRASILIENSIS; ELONGATION-FACTOR; SPINA-BIFIDA; MAJOR ALLERGEN; PROTEINS; SEQUENCE; IDENTIFICATION; PRODUCTS; CLONING; HEV-B-5;
Keywords:
ALLERGENS; ELISA INHIBITION; IGE; LATEX PROTEINS; MEDICAL GLOVES; NATURAL RUBBER LATEX; RAST INHIBITION; SKIN PRICK TEST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
T. Palosuo et al., "MEASUREMENT OF NATURAL-RUBBER LATEX ALLERGEN LEVELS IN MEDICAL GLOVESBY ALLERGEN-SPECIFIC IGE-ELISA INHIBITION, RAST INHIBITION, AND SKIN PRICK TEST", Allergy, 53(1), 1998, pp. 59-67

Abstract

Exposure to natural rubber latex (NRL) medical gloves poses risks to patients sensitized to NRL and to users of protective gloves. Previousstudies have shown that extractable allergen levels of the gloves vary widely Since most of the available laboratory methods of NRL allergen measurement lack adequate validation, we wanted to evaluate the performance of a recently developed competitive IgE-ELISA-inhibition method in relation to the skin prick test (SPT) and RAST inhibition, as well as to extractable protein quantification and an immunochemical latexantigen assay (LEAP). Twenty samples of surgical (n=14) and examination gloves (n=6), covering >90% of medical gloves marketed in Finland in 1994-5, were collected by the Finnish National Research and Development Centre for Welfare and Health, coded, extracted, and analyzed by the five methods. The IgE-ELISA inhibition correlated highly significantly with SPT (r=0.94) and PAST inhibition (r=0.96). Likewise, ELISA inhibition and RAST inhibition showed highly significant correlation (P=0.96, P<0.0001 in all three instances). Protein quantification by a modified Lowry method also correlated highly significantly with SPT (r=0.80), PAST inhibition (r=0.82), and ELISA inhibition (r=0.81, P<0.0001in all three instances), Clearly weaker correlation, though statistically significant (r=0.48, P=0.03), was found between SPT and the LEAP assay An NRL standard preparation was assigned an arbitrary content of100 000 allergen units (AU) per ml. In relation to this standard, theNRL allergen level was considered low (<10 AU/ml) in 11, moderate (10-100 AU/ml) in two, and high (>100 AU/ml) in seven of the 20 glove brands analyzed. In conclusion, the results of a novel IgE-ELISA-inhibition method of measuring NRL allergen levels in medical gloves correlated highly significantly with those of SPT. The ELISA method was found to be sensitive, reproducible, technically easy inexpensive, and suitable for the analysis of large numbers of NRL products. The results of extensive market surveys in 1994 and 1995, communicated to the medical community in Finland, appear to have had a clear effect in moving glove purchasing policies toward the use of low-allergen gloves.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 03:48:44